Western Blot (WB)
(Anti-Ehmt2 Antibody (Center)at 1:1000 dilution + mouse brain lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 138 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Mouse Ehmt2 Polyclonal Antibody | anti-Ehmt2 antibody
(Mouse) Ehmt2 Antibody (Center)
Western Blot (WB)
(Anti-Ehmt2 Antibody (Center)at 1:1000 dilution + mouse brain lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 138 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-Ehmt2 Antibody (Center)at 1:1000 dilution + mouse brain lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 138 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Xie T.,et al.Genome Res. 13:2621-2636(2003).
Church D.M.,et al.PLoS Biol. 7:E1000112-E1000112(2009).
Brown S.E.,et al.Mamm. Genome 12:916-924(2001).
Tachibana M.,et al.J. Biol. Chem. 276:25309-25317(2001).
NCBI and Uniprot Product Information
Uniprot Description
EHMT2: Histone methyltransferase. Preferentially methylates 'Lys-9' of histone H3 and 'Lys-27' of histone H3 (in vitro). H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. Also methylates histone H1. Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with GFI1B, WIZ and EHMT1. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Methyltransferase, protein lysine; Methyltransferase; EC 2.1.1.43; Amino Acid Metabolism - lysine degradation
Cellular Component: nucleoplasm; intermediate filament cytoskeleton; intracellular membrane-bound organelle; nuclear chromatin; nucleolus; chromosome; nucleus
Molecular Function: methyltransferase activity; trimethylamine methyltransferase activity; monomethylamine methyltransferase activity; tRNA (cytosine)-methyltransferase activity; RNA methyltransferase activity; rRNA (cytosine-C5-967)-methyltransferase activity; rRNA (uridine) methyltransferase activity; N-methyltransferase activity; histone-lysine N-methyltransferase activity; tRNA (guanine) methyltransferase activity; histone lysine N-methyltransferase activity (H3-K27 specific); rRNA (adenine-N6,N6-)-dimethyltransferase activity; arginine N-methyltransferase activity; selenocysteine methyltransferase activity; protein-arginine N-methyltransferase activity; methanol-specific methylcobalamin:coenzyme M methyltransferase activity; tRNA (guanine-N2-)-methyltransferase activity; dimethylamine methyltransferase activity; protein methyltransferase activity; 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity; transferase activity; hydroxyneurosporene-O-methyltransferase activity; rRNA (uridine-2'-O-)-methyltransferase activity; tRNA (uracil) methyltransferase activity; rRNA (adenine) methyltransferase activity; histone lysine N-methyltransferase activity (H3-K9 specific); C-methyltransferase activity; cobalt-precorrin-6B C5-methyltransferase activity; cobalt-precorrin-3 C17-methyltransferase activity; 1-phenanthrol methyltransferase activity; zinc ion binding; metal ion binding; S-methyltransferase activity; lysine N-methyltransferase activity; DNA-methyltransferase activity; tRNA (cytosine-5-)-methyltransferase activity; tRNA (adenine)-methyltransferase activity; C-terminal protein carboxyl methyltransferase activity; rRNA (cytosine) methyltransferase activity; cobalt-precorrin-7 C15-methyltransferase activity; protein-arginine N5-methyltransferase activity; rRNA methyltransferase activity; p53 binding; protein-leucine O-methyltransferase activity; demethylmenaquinone methyltransferase activity; rRNA (adenine-N6-)-methyltransferase activity; tRNA methyltransferase activity; rRNA (guanine) methyltransferase activity; methylarsonite methyltransferase activity; mRNA methyltransferase activity; protein binding; methylamine-specific methylcobalamin:coenzyme M methyltransferase activity; tRNA (guanine-N1-)-methyltransferase activity; cobalt-precorrin-5B C1-methyltransferase activity; protein-lysine N-methyltransferase activity; O-methyltransferase activity; tRNA (adenine-N1-)-methyltransferase activity; tRNA (adenine-57, 58-N(1)-) methyltransferase activity
Biological Process: methylation; synaptonemal complex assembly; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; germ cell development; peptidyl-lysine methylation; regulation of transcription from RNA polymerase II promoter; histone methylation; organ growth; cytosine methylation within a CG sequence; fertilization; long-term memory; peptidyl-lysine di-methylation; DNA methylation; regulation of histone H3-K4 methylation; regulation of histone H3-K9 methylation; spermatid development; regulation of DNA replication; histone H3-K9 methylation
Research Articles on Ehmt2
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Product Notes
The Ehmt2 ehmt2 (Catalog #AAA9209286) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The (Mouse) Ehmt2 Antibody (Center) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Ehmt2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the Ehmt2 ehmt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ehmt2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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