Rabbit anti-Mouse Ehmt2 Polyclonal Antibody | anti-Ehmt2 antibody

(Mouse) Ehmt2 Antibody (Center)

Cat. Num. AAA9209286

• Gene Names: Ehmt2; G9a; Bat8; NG36; KMT1C; D17Ertd710e
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Ehmt2; Polyclonal Antibody; (Mouse) Ehmt2 Antibody (Center); Histone-lysine N-methyltransferase EHMT2; 211-; Euchromatic histone-lysine N-methyltransferase 2; HLA-B-associated transcript 8; Histone H3-K9 methyltransferase 3; H3-K9-HMTase 3; Protein G9a; Bat8; G9a; Ng36; anti-Ehmt2 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This Mouse Ehmt2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 589-623 amino acids from the Central region of Mouse Ehmt2.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 1,206

• Applicable Applications for anti-Ehmt2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-Ehmt2 Antibody (Center)at 1:1000 dilution + mouse brain lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 138 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-Ehmt2 antibody

Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys- 373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.

Product Categories/Family for anti-Ehmt2 antibody

Stem Cells

References

Tachibana M.,et al.Genes Dev. 16:1779-1791(2002).
Xie T.,et al.Genome Res. 13:2621-2636(2003).
Church D.M.,et al.PLoS Biol. 7:E1000112-E1000112(2009).
Brown S.E.,et al.Mamm. Genome 12:916-924(2001).
Tachibana M.,et al.J. Biol. Chem. 276:25309-25317(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 557440749
• NCBI GeneID: 110147
• NCBI Accession #: NP_001273502.1
• NCBI GenBank Nucleotide #: NM_001286573.1
• UniProt Accession #: Q9Z148; Q6PE08; Q8K4R6; Q8K4R7; Q9Z149; A2CG75
• Molecular Weight: 138039
• NCBI Official Full Name: histone-lysine N-methyltransferase EHMT2 isoform c
• NCBI Official Synonym Full Names: euchromatic histone lysine N-methyltransferase 2
• NCBI Official Symbol: Ehmt2
• NCBI Official Synonym Symbols: G9a; Bat8; NG36; KMT1C; D17Ertd710e
• NCBI Protein Information: histone-lysine N-methyltransferase EHMT2
• UniProt Protein Name: Histone-lysine N-methyltransferase EHMT2
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: Ehmt2
• UniProt Synonym Gene Names: Bat8; G9a; Ng36; H3-K9-HMTase 3
• UniProt Entry Name: EHMT2_MOUSE

Uniprot Description

EHMT2: Histone methyltransferase. Preferentially methylates 'Lys-9' of histone H3 and 'Lys-27' of histone H3 (in vitro). H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. Also methylates histone H1. Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with GFI1B, WIZ and EHMT1. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase, protein lysine; Methyltransferase; EC 2.1.1.43; Amino Acid Metabolism - lysine degradation

Cellular Component: nucleoplasm; intermediate filament cytoskeleton; intracellular membrane-bound organelle; nuclear chromatin; nucleolus; chromosome; nucleus

Molecular Function: methyltransferase activity; trimethylamine methyltransferase activity; monomethylamine methyltransferase activity; tRNA (cytosine)-methyltransferase activity; RNA methyltransferase activity; rRNA (cytosine-C5-967)-methyltransferase activity; rRNA (uridine) methyltransferase activity; N-methyltransferase activity; histone-lysine N-methyltransferase activity; tRNA (guanine) methyltransferase activity; histone lysine N-methyltransferase activity (H3-K27 specific); rRNA (adenine-N6,N6-)-dimethyltransferase activity; arginine N-methyltransferase activity; selenocysteine methyltransferase activity; protein-arginine N-methyltransferase activity; methanol-specific methylcobalamin:coenzyme M methyltransferase activity; tRNA (guanine-N2-)-methyltransferase activity; dimethylamine methyltransferase activity; protein methyltransferase activity; 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity; transferase activity; hydroxyneurosporene-O-methyltransferase activity; rRNA (uridine-2'-O-)-methyltransferase activity; tRNA (uracil) methyltransferase activity; rRNA (adenine) methyltransferase activity; histone lysine N-methyltransferase activity (H3-K9 specific); C-methyltransferase activity; cobalt-precorrin-6B C5-methyltransferase activity; cobalt-precorrin-3 C17-methyltransferase activity; 1-phenanthrol methyltransferase activity; zinc ion binding; metal ion binding; S-methyltransferase activity; lysine N-methyltransferase activity; DNA-methyltransferase activity; tRNA (cytosine-5-)-methyltransferase activity; tRNA (adenine)-methyltransferase activity; C-terminal protein carboxyl methyltransferase activity; rRNA (cytosine) methyltransferase activity; cobalt-precorrin-7 C15-methyltransferase activity; protein-arginine N5-methyltransferase activity; rRNA methyltransferase activity; p53 binding; protein-leucine O-methyltransferase activity; demethylmenaquinone methyltransferase activity; rRNA (adenine-N6-)-methyltransferase activity; tRNA methyltransferase activity; rRNA (guanine) methyltransferase activity; methylarsonite methyltransferase activity; mRNA methyltransferase activity; protein binding; methylamine-specific methylcobalamin:coenzyme M methyltransferase activity; tRNA (guanine-N1-)-methyltransferase activity; cobalt-precorrin-5B C1-methyltransferase activity; protein-lysine N-methyltransferase activity; O-methyltransferase activity; tRNA (adenine-N1-)-methyltransferase activity; tRNA (adenine-57, 58-N(1)-) methyltransferase activity

Biological Process: methylation; synaptonemal complex assembly; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; germ cell development; peptidyl-lysine methylation; regulation of transcription from RNA polymerase II promoter; histone methylation; organ growth; cytosine methylation within a CG sequence; fertilization; long-term memory; peptidyl-lysine di-methylation; DNA methylation; regulation of histone H3-K4 methylation; regulation of histone H3-K9 methylation; spermatid development; regulation of DNA replication; histone H3-K9 methylation