Testing Data
(Staining of mouse spleen cells with Hamster anti Mouse TCR alpha/beta (MBS212135))
Hamster TCR ALPHA/BETA Monoclonal Antibody
HAMSTER ANTI MOUSE TCR ALPHA/BETA BETA CHAIN:FITC
Applications
Flow Cytometry, Functional Assay
Synonyms
TCR ALPHA/BETA; Monoclonal Antibody; HAMSTER ANTI MOUSE TCR ALPHA/BETA BETA CHAIN:FITC; anti-TCR ALPHA/BETA antibody
Host
Hamster
Clonality
Monoclonal
Isotype
IgG
Clone Number
H57-597
Form/Format
FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Concentration
IgG concentration 0.5 mg/ml (varies by lot)
Applicable Applications for anti-TCR ALPHA/BETA antibody
Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR.
Flow Cytometry: Maximum Dilution: Neat
Flow Cytometry: Maximum Dilution: Neat
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation
1% Bovine Serum Albumin
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Mouse
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data
(Staining of mouse spleen cells with Hamster anti Mouse TCR alpha/beta (MBS212135))
Testing Data
(Staining of mouse spleen cells with Hamster anti Mouse TCR alpha/beta (MBS212135))
Testing Data
(Staining of mouse spleen cells with Hamster anti Mouse TCR Alpha/Beta:FITC (MBS210680))
Testing Data
(Staining of mouse spleen cells with Hamster anti Mouse TCR Alpha/Beta:FITC (MBS210680))
Related Product Information for anti-TCR ALPHA/BETA antibody
Hamster anti mouse TCR alpha/beta, clone H57-597, recognises the beta-chain of the murine alpha/beta T cell receptor and does not show any reactivity to TCR gamma /delta (Gascoigne, N., 1990). Clone H57-597 has been reported to activate T-cells in immobilised form (Kubo, R. et al., 1989) and has also been used for in vivo cell depletion (van der Heyde, H. et al., 1995 and Skeen, M. & Ziegler, H., 1993). Removal of sodium azide is recommended prior to using this clone in functional assays. MyBioSource recommend the Slide-A-Lyzer Dialysis Cassette for this purpose.
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Product Notes
The TCR ALPHA/BETA (Catalog #AAA210681) is an Antibody produced from Hamster and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TCR ALPHA/BETA can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR. Flow Cytometry: Maximum Dilution: Neat. Researchers should empirically determine the suitability of the TCR ALPHA/BETA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCR ALPHA/BETA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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