Goat SMARCA4 / BRG1 Polyclonal Antibody | anti-SMARCA4 antibody

Goat Polyclonal to Human SMARCA4 / BRG1

Cat. Num. AAA244480

• Gene Names: SMARCA4; BRG1; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A
• Reactivity: Bat, Gibbon, Chicken, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat; Predicted Reactivity: Bovine, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: SMARCA4 / BRG1; Polyclonal Antibody; Goat Polyclonal to Human SMARCA4 / BRG1; Anti-SMARCA4 / BRG1 Antibody (aa2-14) IHC-plus; SMARCA4; ATP-dependent helicase SMARCA4; BAF190; BRG1-associated factor 190A; Brahma protein-like 1; BRG1; BRM/SWI2-related gene 1; HSNF2b; MRD16; Protein BRG-1; SNF2-like 4; SNF2LB; SNF2-BETA; SNF2B; SWI2; Nuclear protein GRB1; SNF2; SNF2L4; Human SMARCA4; anti-SMARCA4 antibody

• Host: Goat
• Reactivity: Bat, Gibbon, Chicken, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat; Predicted Reactivity: Bovine, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human SMARCA4 / BRG1. This antibody is expected to recognize all reported isoforms (NP_001122321.1; NP_003063.2; NP_001122317.1; NP_001122318.1; NP_001122319.1; NP_001122320.1). Reported variants NP_003063.2 and NP_001122316.1 represent identical protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 1647

• Applicable Applications for anti-SMARCA4 antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (3.75 - 7.5 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic peptide STPDPPLGGTPRP-C from the N-terminus of human SMARCA4 / BRG1 (NP_001122321.1; NP_003063.2; NP_001122317.1; NP_001122318.1; NP_001122319.1; NP_001122320.1).
• Immunogen Type: Synthetic peptide
• Immunogen: SMARCA4 / BRG1 antibody was raised against synthetic peptide STPDPPLGGTPRP-C from the N-terminus of human SMARCA4 / BRG1 (NP_001122321.1; NP_003063.2; NP_001122317.1; NP_001122318.1; NP_001122319.1; NP_001122320.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Horse, Rabbit, Pig, Opossum, Chicken, Platypus (100%); Bovine, Xenopus, Stickleback, Pufferfish, Zebrafish (92%).
• Antigen Modification: aa2-14
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-SMARCA4 / BRG1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Immunohistochemistry (IHC)

(Anti-SMARCA4 / BRG1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Immunohistochemistry (IHC)

(Anti-SMARCA4 / BRG1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 21071056
• NCBI GeneID: 6597
• NCBI Accession #: NP_003063.2
• NCBI GenBank Nucleotide #: NM_003072.3
• UniProt Accession #: P51532; O95052; Q9HBD3; B1A8Z4; B1A8Z5; B1A8Z6; B1A8Z7; E9PBR8
• Molecular Weight: 181,261 Da
• NCBI Official Full Name: transcription activator BRG1 isoform B
• NCBI Official Synonym Full Names: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
• NCBI Official Symbol: SMARCA4
• NCBI Official Synonym Symbols: BRG1; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A
• NCBI Protein Information: transcription activator BRG1; SNF2-beta; SNF2-like 4; protein BRG-1; nuclear protein GRB1; brahma protein-like 1; BRM/SWI2-related gene 1; homeotic gene regulator; protein brahma homolog 1; BRG1-associated factor 190A; sucrose nonfermenting-like 4; ATP-dependent helicase SMARCA4; mitotic growth and transcription activator; global transcription activator homologous sequence
• UniProt Protein Name: Transcription activator BRG1
• Protein Family: Transcription activator
• UniProt Gene Name: SMARCA4
• UniProt Synonym Gene Names: BAF190A; BRG1; SNF2B; SNF2L4; BAF190A
• UniProt Entry Name: SMCA4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

Uniprot Description

SMARCA4: a member of the SWI/SNF nucleosome remodeling complex with ATP-dependent DNA helicase activity. SWI/SNF complexes are required for mammalian development and are mutated in ~20% of all human primary tumors. Mutated in 35% (13/37) of NSCLC and 5% (1/19) of SCLC human lung cancer cell lines . A component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. Belongs to the neural progenitor-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). Component of the BAF53 complex which acetylates histone H4 and H2A within nucleosomes. Transcriptional coactivator of nuclear hormone receptors. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription, and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1. Can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. SMARCA4, in consort with ZEB1, represses the transcription of E-cadherin and promotes the epithelial-to-mesenchymal transisition (EMT). SMARCA4 is required for stem cell maintenance in the mouse intestinal epithelium. Its loss inhibits aberrant Wnt-signalling and prevents tumorigenesis in the mouse small intestine. SMARCA4 mutations are the cause of a familial cancer syndrome predisposing to renal or extrarenal malignant rhabdoid tumors and to a variety of tumors of the central nervous system, including choroid plexus carcinoma, medulloblastoma, and central primitive neuroectodermal tumors. Rhabdoid tumors are the most aggressive and lethal malignancies occurring in early childhood. Belongs to the SNF2/RAD54 helicase family. The human protein includes 5 isoforms produced by alternative splicing.

Protein type: Cell cycle regulation; Nuclear receptor co-regulator; Tumor suppressor; Transcription, coactivator/corepressor; EC 3.6.4.-; EC 3.6.1.-; Helicase

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; heterochromatin; SWI/SNF complex; extracellular space; protein complex; perichromatin fibrils; membrane; nuclear chromatin; nucleolus; nucleus

Molecular Function: DNA-dependent ATPase activity; protein binding; nucleosomal DNA binding; androgen receptor binding; Tat protein binding; p53 binding; transcription coactivator activity; protein N-terminus binding; helicase activity; transcription corepressor activity; ATP binding

Biological Process: extracellular matrix organization and biogenesis; establishment and/or maintenance of chromatin architecture; glial cell fate determination; negative regulation of transcription from RNA polymerase II promoter, mitotic; positive regulation of transcription, DNA-dependent; cell morphogenesis; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; embryonic organ morphogenesis; epidermis morphogenesis; forebrain development; blastocyst hatching; blastocyst growth; vasculogenesis; positive regulation of Wnt receptor signaling pathway; hindbrain development; positive regulation of DNA binding; transcription, DNA-dependent; stem cell maintenance; liver development; regulation of transcription from RNA polymerase II promoter; chromatin remodeling; keratinocyte differentiation; cytosine methylation within a CG sequence; methylation-dependent chromatin silencing; nucleosome disassembly; ATP-dependent chromatin remodeling; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; negative regulation of transcription, DNA-dependent

Disease: Mental Retardation, Autosomal Dominant 16; Rhabdoid Tumor Predisposition Syndrome 2