Rabbit anti-Human CHML Polyclonal Antibody | anti-CHML antibody

Rabbit Polyclonal (IgG) to Human CHML

Cat. Num. AAA247980

• Gene Names: CHML; REP2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: CHML; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human CHML; Anti-CHML Antibody (aa128-177) IHC-plus; Rab escort protein 2; REP-2; Choroideraemia-like protein; REP2; Human CHML; anti-CHML antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CHML Antibody detects endogenous levels of total CHML protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 656

• Applicable Applications for anti-CHML antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:1000), IHC-P (5 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human CHML (aa128-177).
• Immunogen: CHML antibody was raised against synthetic peptide from human CHML (aa128-177).
• Antigen Modification: aa128-177
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-CHML antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.)

Western Blot (WB)

(Western blot of extracts from COLO cells, using CHML Antibody. The lane on the right is treated with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 53832014
• NCBI GeneID: 1122
• NCBI Accession #: NP_001812.2
• NCBI GenBank Nucleotide #: NM_001821.3
• UniProt Accession #: P26374; Q17RE0; Q9H1Y4; B2RAB9
• Molecular Weight: 74,071 Da
• NCBI Official Full Name: rab proteins geranylgeranyltransferase component A 2
• NCBI Official Synonym Full Names: choroideremia-like (Rab escort protein 2)
• NCBI Official Symbol: CHML
• NCBI Official Synonym Symbols: REP2
• NCBI Protein Information: rab proteins geranylgeranyltransferase component A 2; choroideremia-like protein; choroideraemia-like protein
• UniProt Protein Name: Rab proteins geranylgeranyltransferase component A 2
• Protein Family: Rab proteins geranylgeranyltransferase component
• UniProt Gene Name: CHML
• UniProt Synonym Gene Names: REP2; REP-2
• UniProt Entry Name: RAE2_HUMAN

NCBI Description

The product of the CHML gene supports geranylgeranylation of most Rab proteins and may substitute for REP-1 in tissues other than retina. CHML is localized close to the gene for Usher syndrome type II. [provided by RefSeq, Jul 2008]

Uniprot Description

CHML: Binds unprenylated Rab proteins, presents it to the catalytic Rab GGTase dimer, and remains bound to it after the geranylgeranyl transfer reaction. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Less effective than REP-1 in supporting prenylation of Rab3 family. Belongs to the Rab GDI family.

Chromosomal Location of Human Ortholog: 1q43

Cellular Component: nucleoplasm; Rab-protein geranylgeranyltransferase complex; cytoplasm; cytosol

Molecular Function: Rab GTPase binding; GTPase activator activity; Rab-protein geranylgeranyltransferase activity

Biological Process: protein geranylgeranylation; intracellular protein transport; positive regulation of GTPase activity