Testing Data
(Staining of rat splenocytes with Mouse anti Rat TCY alpha/beta:FITC)
Mouse TCR ALPHA/BETA Monoclonal Antibody
MOUSE ANTI RAT TCR ALPHA/BETA:FITC
Applications
Flow Cytometry, Functional Assay
Synonyms
TCR ALPHA/BETA; Monoclonal Antibody; MOUSE ANTI RAT TCR ALPHA/BETA:FITC; anti-TCR ALPHA/BETA antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
R73
Form/Format
FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Concentration
IgG concentration 0.1 mg/ml (varies by lot)
Applicable Applications for anti-TCR ALPHA/BETA antibody
Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 lymphocytes in 100ul.
Flow Cytometry: Maximum Dilution: Neat
Flow Cytometry: Maximum Dilution: Neat
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation
1% Bovine Serum Albumin
Preparation
Immunogen
Rat T Blasts and Erythrocytes
Fusion Partners
Fusion Partners
Target Species
Rat
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data
(Staining of rat splenocytes with Mouse anti Rat TCY alpha/beta:FITC)
Testing Data
(Staining of rat splenocytes with Mouse anti Rat TCY alpha/beta:FITC)
Testing Data
(Staining of rat spleen cells with Mouse anti Rat TCR alpha/beta:Low Endotoxin (MBS211259))
Testing Data
(Staining of rat spleen cells with Mouse anti Rat TCR alpha/beta:Low Endotoxin (MBS211259))
Related Product Information for anti-TCR ALPHA/BETA antibody
Mouse anti Rat TCR alpha/beta antibody, clone R73 recognizes a constant determinant on the beta chain of the rat alpha/beta T cell receptor, expressed by 97% of peripheral rat T cells as defined by the OX-52 marker. R73 is mitogenic for unseparated spleen cells and for purified T cells. In the rat thymus, mature medullary cells express the R73 determinant at the same levels as peripheral T cells, whereas 94% of CD4 - CD8 - thymocytes are R73 negative. Mouse anti Rat TCR alpha/beta antibody, clone R73 is reported to stimulate adhesion between Thymic Dendritic Cells and Thymocytes (Colic et al. 2010).
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Product Notes
The TCR ALPHA/BETA (Catalog #AAA211018) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TCR ALPHA/BETA can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 lymphocytes in 100ul. Flow Cytometry: Maximum Dilution: Neat. Researchers should empirically determine the suitability of the TCR ALPHA/BETA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCR ALPHA/BETA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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