Testing Data
(Blots of crude HeLa cell homogenate blotted with MBS6011548. The MBS6011548 monoclonal binds strongly and cleanly to a band at about 68kD.)
Mouse anti-Human TATA-binding Protein-associated Factor 2N Monoclonal Antibody | anti-Taf15 antibody
TATA-binding Protein-associated Factor 2N (68 kD TATA-binding Protein-associated Factor, TAF(II)68, TAFII68, RNA-binding Protein 56, RBP56, TAF15, TAF2N)
Gene Names
Taf15; 68kDa; Taf2n; TAFII68; 2610111C21Rik
Reactivity
Human
Applications
Western Blot, Immunofluorescence
Purity
AscitesPurified by Protein G affinity chromatography.
Synonyms
TATA-binding Protein-associated Factor 2N; Monoclonal Antibody; TATA-binding Protein-associated Factor 2N (68 kD TATA-binding Protein-associated Factor; TAF(II)68; TAFII68; RNA-binding Protein 56; RBP56; TAF15; TAF2N); Anti -TATA-binding Protein-associated Factor 2N (68 kD TATA-binding Protein-associated Factor; anti-Taf15 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
13B863
Specificity
Recognizes TAF15 specifically both in western blots and in immunocytochemical experiments. On blots it eveals a prominent 68 kD band, and on cells in tissue culture the antibody stains mainly in nucleus.
Purity/Purification
Ascites
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.05% sodium azide.
Applicable Applications for anti-Taf15 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence and Western Blot.
Dilution: Immunofluorescence: 1:500-1:1000
Western Blot: 1:1000; A suitable control tissue is HeLa cell lysates.
Dilution: Immunofluorescence: 1:500-1:1000
Western Blot: 1:1000; A suitable control tissue is HeLa cell lysates.
Immunogen
Full length recombinant human TAF15 expressed in and purified from E. coli.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Testing Data
(Blots of crude HeLa cell homogenate blotted with MBS6011548. The MBS6011548 monoclonal binds strongly and cleanly to a band at about 68kD.)
Testing Data
(Blots of crude HeLa cell homogenate blotted with MBS6011548. The MBS6011548 monoclonal binds strongly and cleanly to a band at about 68kD.)
Testing Data
(Shows HeLa cell cultures stained with MBS6011548 antibody (green) and chicken antibody to vimentin 031441 (red). MBS6011548 reveals a granular nuclear localization typical of many RNA associated proteins. Blue is a DNA stain.)
Testing Data
(Shows HeLa cell cultures stained with MBS6011548 antibody (green) and chicken antibody to vimentin 031441 (red). MBS6011548 reveals a granular nuclear localization typical of many RNA associated proteins. Blue is a DNA stain.)
Related Product Information for anti-Taf15 antibody
TAF15 is a member of a family of 3 closely related mammalian RNA binding proteins, the other members being FUS/TLS and EWSR1. TDP43 is a more distant relative of these three. The protein is also known as RBP56, for RNA binding protein 56kD (1). The Human Genome Organization (HUGO) Gene Nomenclature Committee (HGNC) name for this protein is TAF15. As with the other members of this family, TAF15 contains one copy of the ~90 amino acid RRM domain. RRM is an acronym for RNA Recognition Motif, and this domain is found in many proteins which bind single stranded RNA and DNA. It also has a single Zinc Finger domain of the ZnF_RBZ subtype found in Ran binding proteins. Ran is a small G protein related to p21-Ras which regulates the import and export of proteins to the nucleus. Like TDP43, Fus/TLS and EWS, TAF15 is widely expressed in tissues and is localized primarily in the nucleus of cells (2). It was recently shown that point mutations in TAF15 may be associated with some familial forms of Lou Gehrig's disease, also known as amyotrophic lateral sclerosis or ALS (3). Fus/TLS and TDP43 protein mutations and aggregation have been implicated in various kinds of neurological disease including ALS (4). The HGNC name for this protein is TAF15.
Product Categories/Family for anti-Taf15 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
TATA-binding protein-associated factor 2N
NCBI Official Synonym Full Names
TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor
NCBI Official Symbol
Taf15
NCBI Official Synonym Symbols
68kDa; Taf2n; TAFII68; 2610111C21Rik
NCBI Protein Information
TATA-binding protein-associated factor 2N; TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68 kDa; TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD (RNA binding protein 56)
Protein Family
Research Articles on Taf15
Similar Products
Product Notes
The Taf15 (Catalog #AAA6011548) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TATA-binding Protein-associated Factor 2N (68 kD TATA-binding Protein-associated Factor, TAF(II)68, TAFII68, RNA-binding Protein 56, RBP56, TAF15, TAF2N) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TATA-binding Protein-associated Factor 2N can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Suitable for use in Immunofluorescence and Western Blot. Dilution: Immunofluorescence: 1:500-1:1000 Western Blot: 1:1000; A suitable control tissue is HeLa cell lysates. Researchers should empirically determine the suitability of the Taf15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TATA-binding Protein-associated Factor 2N, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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