Rabbit anti-Human TAF9B Polyclonal Antibody | anti-TAF9B antibody

TAF9B, NT (TAF9B, TAF9L, Transcription initiation factor TFIID subunit 9B, Neuronal cell death-related protein 7, Transcription initiation factor TFIID subunit 9-like, Transcription-associated factor TAFII31L) (APC)

Cat. Num. AAA6353458

• Gene Names: TAF9B; DN7; DN-7; TAF9L; TAFII31L; TFIID-31
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TAF9B; Polyclonal Antibody; NT (TAF9B; TAF9L; Transcription initiation factor TFIID subunit 9B; Neuronal cell death-related protein 7; Transcription initiation factor TFIID subunit 9-like; Transcription-associated factor TAFII31L) (APC); Transcription-associated factor TAFII31L; anti-TAF9B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-TAF9B antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TAF9B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-72 amino acids from the N-terminal region of human TAF9B.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TAF9B Antibody (N-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the TAF9B antibody detected the TAF9B protein (arrow).)

Related Product Information for anti-TAF9B antibody

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a protein that is similar to one of the small subunits of TFIID, TBP-associated factor 9, and is also a subunit of TFIID. TAF9 and TAF9b share some functions but also have distinct roles in the transcriptional regulatory process.

Product Categories/Family for anti-TAF9B antibody

Antibodies; Transcription Factors

References

Frontini, M., et al. Mol. Cell. Biol. 25(11):4638-4649(2005). Ross, M.T., et al. Nature 434(7031):325-337(2005). Chen, Z., et al. J. Biol. Chem. 278(37):35172-35183(2003). Albright, S.R., et al. Gene 242 (1-2), 1-13 (2000): Lu, H., et al. Proc. Natl. Acad. Sci. U.S.A. 92(11):5154-5158(1995).

NCBI and Uniprot Product Information

• NCBI GI #: 85062626
• NCBI GeneID: 51616
• NCBI Accession #: NP_057059.2
• NCBI GenBank Nucleotide #: NM_015975
• UniProt Accession #: Q9HBM6; Q9Y2S3; B2RUZ9
• Molecular Weight: 27,622 Da
• NCBI Official Full Name: Homo sapiens TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa (TAF9B), mRNA
• NCBI Official Synonym Full Names: TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa
• NCBI Official Symbol: TAF9B
• NCBI Official Synonym Symbols: DN7; DN-7; TAF9L; TAFII31L; TFIID-31
• NCBI Protein Information: transcription initiation factor TFIID subunit 9B; TAF9-like RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa; TBP-associated factor 9L; neuronal cell death-related protein 7; transcription associated factor TAFII31L; transcriptio
• UniProt Protein Name: Transcription initiation factor TFIID subunit 9B
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF9B
• UniProt Synonym Gene Names: TAF9L
• UniProt Entry Name: TAF9B_HUMAN

NCBI Description

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a protein that is similar to one of the small subunits of TFIID, TBP-associated factor 9, and is also a subunit of TFIID. TAF9 and TAF9b share some functions but also have distinct roles in the transcriptional regulatory process. [provided by RefSeq, Jul 2008]

Uniprot Description

TAF9B: Essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. May have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription. Belongs to the TAF9 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: Xq13.1-q21.1

Cellular Component: nucleoplasm; transcription factor TFIID complex

Molecular Function: protein binding; protein heterodimerization activity; transcription corepressor activity

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; protein stabilization; RNA elongation from RNA polymerase II promoter; gene expression; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell growth; negative regulation of apoptosis