Mouse T LYMPHOCYTES Monoclonal Antibody
MOUSE ANTI RABBIT T LYMPHOCYTES
Applications
Immunohistochemistry, ELISA, Flow Cytometry, Functional Assay, Immunoprecipitation
Synonyms
T LYMPHOCYTES; Monoclonal Antibody; MOUSE ANTI RABBIT T LYMPHOCYTES; anti-T LYMPHOCYTES antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
KEN-5
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Applicable Applications for anti-T LYMPHOCYTES antibody
Immunohistology Frozen, ELISA (EIA), Flow cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/200
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/200
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Rabbit
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Related Product Information for anti-T LYMPHOCYTES antibody
Mouse anti Rabbit T lymphocytes, clone KEN-5, recognizes rabbit (lapine) T-cells. Clone KEN-5 recognizes >90% thymocytes and approximately 40-45% mesenteric lymph node cells and immunoprecipitates a 67 kDa protein from rabbit thymocytes. In contrast to humans and mice where only a very small population of B-cells express the CD5 antigen, the CD5 antigen in rabbits is widely expressed by peripheral blood B cells. However, the KEN-5 antibody, while originally reported as recognising rabbit CD5 (Kotani et al.1993), does not bind to rabbit CD5 transfectants. Furthermore, clone KEN-5, unlike other known antibodies recognising rabbit CD5 and anti human cross reactive CD5 antibodies, shows very limited binding to rabbit B-cells, which in adult rabbits have been demonstrated to express CD5 (Raman & Knight 1992) More recently studies (Pospisil et al. 2009) indicate that clone KEN-5 may recognize a epitope that is dependant on glycosylation to maintain its structural configuration which may explain why this antibody does not recognize recombinant rabbit CD5 produced in insect cells which contain different glycans from those found in mammalian cells. It has therefore been suggested that KEN-5 may distinguish between different isoforms of CD5 in lapine T and B cells (Pospisil et al. 2009).
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Product Notes
The T LYMPHOCYTES (Catalog #AAA219925) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's T LYMPHOCYTES can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, ELISA (EIA), Flow cytometry (FC/FACS), Immunoprecipitation (IP). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/200. Researchers should empirically determine the suitability of the T LYMPHOCYTES for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "T LYMPHOCYTES, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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