Western Blot (WB)
(Western blot analysis of extracts from COS-7, using ATPIF1 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit ATPIF1 Polyclonal Antibody | anti-ATPIF1 antibody
ATPIF1 Antibody
Predicted: Pig, Bovine
Predicted: Pig, Bovine
Western Blot (WB)
(Western blot analysis of extracts from COS-7, using ATPIF1 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from COS-7, using ATPIF1 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using ATPIF1 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using ATPIF1 Antibody. The lane on the left was treated with blocking peptide.)
Post Translational Modifications: Exhibits variability in chain length, mitochondria have distinct pools of protein cleaved after the 24th, 25th, and 26th amino acid.
Subunit Structure: Homodimer; represents the active form and is present at a pH value below 6.5. Homotetramer; represents the inactive form and is present at a pH value above 7.0 (By similarity).
Similarity: Forms an alpha-helical dimer with monomers associated via an antiparallel alpha-helical coiled coil composed of residues 74-106, leaving each N-terminal inhibitory region (residues 26-52) accessible for interaction with an F1 catalytic domain. The inhibitory N-terminal region (residues 26-52) binds the alpha(ADP-bound)-beta(ADP-bound) (ATP5F1A-ATP5F1B) interface of F1-ATPase, and also contact the central gamma subunit (ATP5F1C). This dimeric state is favored by pH values below 7.0, and at higher values the dimers associate to form inactive homotetramer, where the inhibitory region is occluded, masking its inhibitory activity (By similarity). Belongs to the ATPase inhibitor family.
NCBI and Uniprot Product Information
Predicted: 13 kDa
NCBI Description
This gene encodes a mitochondrial ATPase inhibitor. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
ATPIF1: Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Homodimer at a pH value below 6.5, inactive homotetramer above. Belongs to the ATPase inhibitor family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Inhibitor; Mitochondrial
Chromosomal Location of Human Ortholog: 1p35.3
Cellular Component: cell surface; mitochondrion; mitochondrial proton-transporting ATP synthase complex
Molecular Function: calmodulin binding; enzyme inhibitor activity; angiostatin binding; protein homodimerization activity; ATPase inhibitor activity; ATPase binding
Biological Process: generation of precursor metabolites and energy; regulation of gene expression; negative regulation of hydrolase activity; negative regulation of endothelial cell proliferation; erythrocyte differentiation; mitochondrial depolarization; angiogenesis; negative regulation of nucleotide metabolic process; protein homooligomerization; heme biosynthetic process; protein homotetramerization
Research Articles on ATPIF1
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Product Notes
The ATPIF1 atpif1 (Catalog #AAA9611597) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATPIF1 Antibody reacts with Human, Mouse, Monkey Predicted: Pig, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's ATPIF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the ATPIF1 atpif1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATPIF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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