Mouse anti-Human, Rat Surfactant Protein D, Lectin Domain Monoclonal Antibody | anti-SFTPD antibody

Surfactant Protein D, Lectin Domain (SP-D)

Cat. Num. AAA602496

• Gene Names: SFTPD; SP-D; PSP-D; SFTP4; COLEC7
• Reactivity: Human, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Surfactant Protein D; Lectin Domain; Monoclonal Antibody; Lectin Domain (SP-D); Anti -Surfactant Protein D; anti-SFTPD antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2Q2261
• Specificity: Recognizes an epitope localized in the lectin domain of rat surfactant protein D (SP-D). Reacts with alveolar type II cells, alveolar macrophages, types of Clara cells SP-A+ and SP-A- and extracellular deposits. Does not react with Alveolar type III cells. No reaction on rat skin, colon, kidney or liver. Species Crossreactivity: human.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from buffer, 5mg/ml BSA, 0.09% sodium azide. Reconstitute with 500ul sterile ddH2O.

• Applicable Applications for anti-SFTPD antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry and ELISA.; Dilution: Immunohistochemistry (Use snap frozen or paraffin embedded tissue): Fresh rat tissue: 1:400; fresh human tissue: 1:20. Fixation: Acetone 5 minutes at 4 degree C, paraformaldehyde, paraffin. Tissue pretreatment: Microwave treatment 2 x 5 minutes at 750W for paraffin tissues.; ELISA: Use as a capture antibody.
• Immunogen: Purified rat surfactant protein D from bronchoalveolar lavage.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-SFTPD antibody

Surfactant Protein D is a Ca2+ dependent carbohydrate binding protein with a structure similar to other C-type mammalian lectins. Its molecular weight is 620kD under non-reducing conditions and 43kD under reducing conditions. SP-D enhances the production of oxygen radicals in alveolar macrophages and regulates some actions of SP-A. SP-D is a lung surfactant associated collectin that has been implicated in the innate pulmonary host defense against a wide variety of important bacterial, fungal and viral pathogens.

Product Categories/Family for anti-SFTPD antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 292507
• NCBI GeneID: 6441
• UniProt Accession #: P35247; Q5T0M3; Q6FH08; Q86YK9; Q8TCD8; Q9UCJ2; Q9UCJ3
• Molecular Weight: 37,728 Da
• NCBI Official Full Name: surfactant protein D
• NCBI Official Synonym Full Names: surfactant protein D
• NCBI Official Symbol: SFTPD
• NCBI Official Synonym Symbols: SP-D; PSP-D; SFTP4; COLEC7
• NCBI Protein Information: pulmonary surfactant-associated protein D; collectin 7; collectin-7; OTTHUMP00000019944; lung surfactant protein D; pulmonary surfactant apoprotein; surfactant, pulmonary-associated protein D; surfactant-associated protein, pulmonary 4
• UniProt Protein Name: Pulmonary surfactant-associated protein D
• Protein Family: Pulmonary surfactant-associated protein
• UniProt Gene Name: SFTPD
• UniProt Synonym Gene Names: COLEC7; PSPD; SFTP4
• UniProt Entry Name: SFTPD_HUMAN

NCBI Description

The protein encoded by this gene is part of the innate immune response, protecting the lungs against inhaled microorganisms and chemicals. The encoded protein may also be involved in surfactant metabolism. [provided by RefSeq, Jul 2015]

Uniprot Description

SFTPD: Contributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. Belongs to the SFTPD family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10q22.2-q23.1

Cellular Component: extracellular space; proteinaceous extracellular matrix; collagen; endocytic vesicle; lysosome; extracellular region

Molecular Function: protein binding; carbohydrate binding

Biological Process: receptor-mediated endocytosis; negative regulation of T cell proliferation; positive regulation of phagocytosis; negative regulation of interleukin-2 biosynthetic process; defense response to bacterium; innate immune response; respiratory gaseous exchange; surfactant homeostasis; alveolus development; macrophage chemotaxis; regulation of cytokine production