Mouse anti-Human, Rat Superoxide Dismutase, Mn Monoclonal Antibody | anti-sodA antibody
Superoxide Dismutase, Mn (SOD Mn)
Gene Names
sodA; ECK3901; JW3879
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Superoxide Dismutase; Mn; Monoclonal Antibody; Mn (SOD Mn); Anti -Superoxide Dismutase; anti-sodA antibody
Host
Mouse
Reactivity
Human, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2Q339
Specificity
Recognizes human MnSOD. Species Crossreactivity: rat. Does not react with mouse.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2-7.4.
Applicable Applications for anti-sodA antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot.
Dilution: Immunohistochemistry (Acetone fixed cryostat sections): 0.5-10ug/ml using an APAAP or PAP procedure. Antibody also reacts with 4% PFA fixed frozen tissue. Typical block contains 1% non-fat dry milk or 3% NGS and 0.2-1.5% triton X-100 (higher triton is only used on thick sections; blocked for 1-3 hours at RT. Primary antibody is diluted only in PBS or TBS without excess blocking materials (no triton or milk); typical dilution is 1:200-1:1000 with incubations up to 48 hours at 4 degree C. Wash 3x10' in PBS and detect via DAB. Immunoreactive neurons are characterized by the dark brown, fine granular deposition in soma and proximal dendrites.
Immunocytochemistry (cell smears): 0.5-10ug/ml.
Western Blot: Under reducing conditions a band at ~25kD will be identified.
Dilution: Immunohistochemistry (Acetone fixed cryostat sections): 0.5-10ug/ml using an APAAP or PAP procedure. Antibody also reacts with 4% PFA fixed frozen tissue. Typical block contains 1% non-fat dry milk or 3% NGS and 0.2-1.5% triton X-100 (higher triton is only used on thick sections; blocked for 1-3 hours at RT. Primary antibody is diluted only in PBS or TBS without excess blocking materials (no triton or milk); typical dilution is 1:200-1:1000 with incubations up to 48 hours at 4 degree C. Wash 3x10' in PBS and detect via DAB. Immunoreactive neurons are characterized by the dark brown, fine granular deposition in soma and proximal dendrites.
Immunocytochemistry (cell smears): 0.5-10ug/ml.
Western Blot: Under reducing conditions a band at ~25kD will be identified.
Immunogen
Manganese superoxide dismutase (MnSOD).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-sodA antibody
Superoxide dismutases (SOD) are found in all aerobic organisms. Their physiological function appears to be to protect cells from free radicals by scavenging superoxide anions. They catalyse the dismutation of superoxide anions to oxygen and hydrogen peroxide. SOD(Mn) is located primarily in mitrochondria and is thought to be of prokaryote origin. SOD(Mn) is a tetramer of identical subunits of MW 25kD by SDS-PAGE.
Product Categories/Family for anti-sodA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,097 Da
NCBI Official Full Name
superoxide dismutase, Mn
NCBI Official Symbol
sodA
NCBI Official Synonym Symbols
ECK3901; JW3879
NCBI Protein Information
superoxide dismutase, Mn
UniProt Protein Name
Superoxide dismutase [Mn]
Protein Family
UniProt Gene Name
sodA
UniProt Entry Name
SODM_ECOLI
NCBI Description
SoxRS regulon. [More information is available at EcoGene: EG10953].
Uniprot Description
Function: Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
Catalytic activity: 2 superoxide + 2 H+ = O2 + H2O2.
Cofactor: Binds 1 manganese ion per subunit.
Subunit structure: Homodimer.
Sequence similarities: Belongs to the iron/manganese superoxide dismutase family.
Research Articles on sodA
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Product Notes
The sodA soda (Catalog #AAA602949) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Superoxide Dismutase, Mn (SOD Mn) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Superoxide Dismutase, Mn can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Dilution: Immunohistochemistry (Acetone fixed cryostat sections): 0.5-10ug/ml using an APAAP or PAP procedure. Antibody also reacts with 4% PFA fixed frozen tissue. Typical block contains 1% non-fat dry milk or 3% NGS and 0.2-1.5% triton X-100 (higher triton is only used on thick sections; blocked for 1-3 hours at RT. Primary antibody is diluted only in PBS or TBS without excess blocking materials (no triton or milk); typical dilution is 1:200-1:1000 with incubations up to 48 hours at 4 degree C. Wash 3x10' in PBS and detect via DAB. Immunoreactive neurons are characterized by the dark brown, fine granular deposition in soma and proximal dendrites. Immunocytochemistry (cell smears): 0.5-10ug/ml. Western Blot: Under reducing conditions a band at ~25kD will be identified. Researchers should empirically determine the suitability of the sodA soda for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Superoxide Dismutase, Mn, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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