Mouse SUMO Monoclonal Antibody | anti-SUMO antibody

Anti-Yeast SUMO (Protein A Purified) Antibody

Cat. Num. AAA4156662

• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified
• Synonyms: SUMO; Monoclonal Antibody; Anti-Yeast SUMO (Protein A Purified) Antibody; Yeast SUMO; anti-SUMO antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Specificity: This product is a monoclonal antibody purified from tissue culture supernatant fluid by Protein A chromatography.
• Purity/Purification: Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)

• Applicable Applications for anti-SUMO antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: This monoclonal antibody reacts with yeast SUMO (Smt3) by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. Using the specified conditions, this antibody may recognize other prominent intrinsic bands (UBLs or conjugates). Other intrinsic bands are readily detectable at lower dilutions.
• Immunogen: This antibody was produced in mice by repeated immunizations with fulllength recombinant yeast SUMO protein.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Testing Data

Related Product Information for anti-SUMO antibody

Covalent modification of cellular proteins by the ubiquitin-like modifier SUMO (small ubiquitinlike modifier) regulates various cellular processes, such as nuclear transport, signal transduction, stress responses and cell cycle progression. However, in contrast to ubiquination, sumoylation does not tag proteins for degradation by the 26S proteasome but rather seems to enhance stability or modulate their subcellular compartmentalization. Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class, including parkin, RAD23 and DSK2, are designated ubiquitindomain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. Once covalently attached to cellular targets, SUMO regulates protein:protein and protein:DNA interactions, as well as localization and stability of the target protein. Sumoylation occurs in most eukaryotic systems, and SUMO is highly conserved from yeast to humans. Where invertebrates have only a single SUMO gene termed SMT3, three members of the SUMO family have been identified in vertebrates: SUMO-1 and the close homologues SUMO-2 and SUMO-3. SUMO has been called SMT3 (yeast), sentrin, PIC1, GMP1 and UBL1. SUMO has been shown to bind and regulate mammalian SP-RINGs (such as Mdm2, PIAS and PML), RanGAP1, RanBP2, p53, p73, HIPK2, TEL, c-Jun, Fas, Daxx, TNFRI, Topo-I, Topo-II, WRN, Sp100, IkB-a, Androgen receptor (AR), GLUT1/4, Drosophila Ttk69, Dorsal, CaMK, yeast Septins, and viral CMV-IE1/2, EBV-BZLF1, HPV/BPV-E1. These bindings implicate SUMO in the stabilization of the target proteins and/or their localization to subcellular complexes. SUMO has an apparent molecular weight of ~12 kDa, and human SUMO-1 (a 101 amino acid polypeptide) shares 50% sequence identity with SUMO-2 and SUMO-3 and with yeast SMT3. SUMO and ubiquitin only show about 18% homology, but both possess a common three-dimensional structure characterized by a tightly packed globular fold with b-sheets wrapped around an a-helix.