Mouse STK3 Monoclonal Antibody | anti-STK3 antibody

STK3 (Serine/Threonine Kinase 3, STE20 Homolog)

Cat. Num. AAA609723

• Gene Names: STK3; KRS1; MST2; FLJ90748
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Gel Super Shift Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: STK3; Monoclonal Antibody; STK3 (Serine/Threonine Kinase 3; STE20 Homolog); Anti -STK3 (Serine/Threonine Kinase 3; anti-STK3 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 8J144
• Specificity: Recognizes human STK3.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 1% BSA and 0.05% sodium azide.

• Applicable Applications for anti-STK3 antibody: Western Blot (WB), Gel Shift Assay (GS/EMSA)
• Application Notes: Suitable for use in Dot Blot and Western Blot.
• Immunogen: Partial sequence of recombinant full-length protein to human STK3
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

Product Categories/Family for anti-STK3 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 103471999
• NCBI GeneID: 6788
• NCBI Accession #: NP_006272.2
• NCBI GenBank Nucleotide #: NM_006281.2
• UniProt Accession #: Q13188; Q15445; Q15801; Q96FM6; A8K722; Q8NBU1
• Molecular Weight: 56,301 Da
• NCBI Official Full Name: serine/threonine-protein kinase 3
• NCBI Official Synonym Full Names: serine/threonine kinase 3
• NCBI Official Symbol: STK3
• NCBI Official Synonym Symbols: KRS1; MST2; FLJ90748
• NCBI Protein Information: serine/threonine-protein kinase 3; MST-2; OTTHUMP00000227170; OTTHUMP00000227171; OTTHUMP00000227172; STE20-like kinase MST2; mammalian STE20-like protein kinase 2; serine/threonine-protein kinase Krs-1; serine/threonine kinase 3 (STE20 homolog, yeast); serine/threonine kinase 3 (Ste20, yeast homolog)
• UniProt Protein Name: Serine/threonine-protein kinase 3
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: STK3
• UniProt Synonym Gene Names: MST2
• UniProt Entry Name: STK3_HUMAN

NCBI Description

Protein kinase activation is a frequent response of cells to treatment with growth factors, chemicals, heat shock, or apoptosis-inducing agents. This protein kinase activation presumably allows cells to resist unfavorable environmental conditions. The yeast 'sterile 20' (Ste20) kinase acts upstream of the mitogen-activated protein kinase (MAPK) cascade that is activated under a variety of stress conditions. MST2 was identified as a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand (MIM 134638) (Taylor et al., 1996 [PubMed 8816758]; Lee et al., 2001 [PubMed 11278283]).[supplied by OMIM]

Uniprot Description

MST2: a protein kinase of the STE20 family. Activated by apoptotic signals as well as other stress conditions. Full activation requires both phosphorylation and caspase-mediated cleavage. Following cleavage by caspase-3, undergoes irreversible autophosphorylation. Full-length is mainly unphosphorylated in the rat thymus and cultured cells, whereas caspase-3-cleaved MST2 in apoptotic cells is highly phosphorylated.

Protein type: EC 2.7.11.1; Autophagy; Kinase, protein; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; MST subfamily

Chromosomal Location of Human Ortholog: 8q22.2

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: protein dimerization activity; protein serine/threonine kinase activator activity; protein serine/threonine kinase activity; protein binding; magnesium ion binding; ATP binding; protein kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: central nervous system development; protein stabilization; positive regulation of protein binding; positive regulation of apoptosis; apoptosis; positive regulation of JNK cascade; signal transduction; negative regulation of organ growth; protein amino acid phosphorylation; negative regulation of cell proliferation; positive regulation of protein kinase B signaling cascade; positive regulation of fat cell differentiation; positive regulation of transcription factor activity; neural tube formation