B3GNT6 recombinant protein

B3GNT6 Protein, Human, Recombinant (aa 44-384, His Tag)

Cat. Num. AAA8121595

• Gene Names: B3GNT6; BGnT-6; B3Gn-T6; beta3Gn-T6; beta-1,3-Gn-T6
• Purity: >90% as determined by SDS-PAGE
• Synonyms: B3GNT6; B3GNT6 Protein; Human; Recombinant (aa 44-384; His Tag); Human B3GNT6 Protein (aa 44-384; B3Gn-T6 Protein; beta-1; 3-Gn-T6 Protein; beta3Gn-T6 Protein; BGnT-6 Protein; UDP-GlcNAc:betaGal beta-1; 3-N-acetylglucosaminyltransferase 6; B3GNT6 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH8.0, 10% gly. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gln44-Ser384

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human B3GNT6 (NP_619651.3)(Gln44-Ser384) was fused with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for B3GNT6 recombinant protein

Background: B3GNT6 belongs to the glycosyltransferase 31 family. B3GNT6 play s an important role in the synthesis of mucin-type O-glycans in digestive organs. It catalyzes the transfer of GlcNAc from UDP-GlcNAc to GalNAcalpha1-Ser/Thr (Tn antigen) to form the core 3 structure (GlcNAcbeta1-3GalNAcalpha1-Ser/Thr). Core 3 structure exists in O-glycan which is an important precursor in the biosynthesis of mucin-type glycoproteins. Loss of core 3 could lead to the production of secreted mucins, then bacteria would be inefficiently cleared from the system, and chronic inflammation would be developed, which eventually would result in development of cancer. B3GNT6 gene is a tumor suppressor gene.

References

Hennet T, et al. (1998) Genomic cloning and expression of three murine UDP-galactose: beta-N-acetylglucosamine beta1,3-galactosyltransferase genes. J Biol Chem. 273(1):58-65. Kolbinger F, et al. (1998) Cloning of a human UDP-galactose:2-acetamido-2-deoxy-D-glucose 3beta-galactosyltransferase catalyzing the formation of type 1 chains. J Biol Chem. 273(1): 433-40. Henrissat B, et al. (1997) A classification of nucleotide-diphospho-sugar glycosyltransferases based on amino acid sequence similarities. Biochem J. 326:929-39.

NCBI and Uniprot Product Information

• NCBI GI #: 118918426
• NCBI GeneID: 192134
• NCBI Accession #: NP_619651.3
• NCBI GenBank Nucleotide #: NM_138706.4
• UniProt Accession #: Q6ZMB0; Q6ZSC5; Q8TAZ4; Q8TDX1; E9PKS1
• Molecular Weight: 42,748 Da
• NCBI Official Full Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6
• NCBI Official Synonym Full Names: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6 (core 3 synthase)
• NCBI Official Symbol: B3GNT6
• NCBI Official Synonym Symbols: BGnT-6; B3Gn-T6; beta3Gn-T6; beta-1,3-Gn-T6
• NCBI Protein Information: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6; core 3 synthase; beta-1,3-N-acetylglucosaminyltransferase protein
• UniProt Protein Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6
• Protein Family: Acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase
• UniProt Gene Name: B3GNT6
• UniProt Synonym Gene Names: BGnT-6; Beta-1,3-Gn-T6; Beta-1,3-N-acetylglucosaminyltransferase 6; Beta3Gn-T6
• UniProt Entry Name: B3GN6_HUMAN

Uniprot Description

B3GNT6: Beta-1,3-N-acetylglucosaminyltransferase that synthesizes the core 3 structure of the O-glycan, an important precursor in the biosynthesis of mucin-type glycoproteins. Plays an important role in the synthesis of mucin-type O-glycans in digestive organs. Belongs to the glycosyltransferase 31 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; Membrane protein, integral; Glycan Metabolism - O-glycan biosynthesis; EC 2.4.1.-

Chromosomal Location of Human Ortholog: 11q13.4

Cellular Component: Golgi membrane; membrane; integral to membrane

Molecular Function: beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase activity; galactosyltransferase activity

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; glycoprotein biosynthetic process; O-glycan processing; O-glycan processing, core 3; post-translational protein modification