Western Blot (WB)
(Figure 1: Western blot analysis using ST13 mouse mAb against A431 (1), HEK293 (2), Hela (3), HepG2 (4), Jurkat (5), K562 (6), L121O (7) and MCF-7 (8) cell lysate.)
Mouse ST13 Monoclonal Antibody | anti-ST13 antibody
ST13 Antibody
Gene Names
ST13; HIP; HOP; P48; AAG2; SNC6; HSPABP; FAM10A1; FAM10A4; HSPABP1; PRO0786
Reactivity
Human, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
Affinity-Chromatography
Synonyms
ST13; Monoclonal Antibody; ST13 Antibody; AAG 2; AAG2; Aging associated protein 2; F10A1_HUMAN; FAM10A1; FAM10A4; FLJ27260; Heat shock 70kD protein binding protein; Hip; HOP; Hsc70 interacting protein; Hsc70-interacting protein; Hsp70 interacting protein; HSPABP 1; HSPABP; HSPABP1; MGC129952; OTTHUMP00000028873; P48; PRO0786; Progesterone receptor associated p48 protein; Progesterone receptor-associated p48 protein; Protein FAM10A1; Putative tumor suppressor ST13; Renal carcinoma antigen NY REN 33; Renal carcinoma antigen NY-REN-33; SNC 6; SNC6; ST 13; St13; Suppression of tumorigenicity 13; Suppression of tumorigenicity 13 (colon carcinoma); Suppression of tumorigenicity 13 protein; Suppression of tumorigenicity protein 13; anti-ST13 antibody
Host
Mouse
Reactivity
Human, Monkey
Clonality
Monoclonal
Isotype
IgG1
Specificity
ST13 antibody detects endogenous levels of total ST13
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
369
Applicable Applications for anti-ST13 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
ELISA: 1:10000
WB: 1:500-1:2000
IHC: 1:200-1:1000
ICC: 1:200-1:1000
WB: 1:500-1:2000
IHC: 1:200-1:1000
ICC: 1:200-1:1000
Immunogen
Purified recombinant fragment of human ST13 expressed in E. Coli
Subcellular Location
Cytoplasm.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Figure 1: Western blot analysis using ST13 mouse mAb against A431 (1), HEK293 (2), Hela (3), HepG2 (4), Jurkat (5), K562 (6), L121O (7) and MCF-7 (8) cell lysate.)
Western Blot (WB)
(Figure 1: Western blot analysis using ST13 mouse mAb against A431 (1), HEK293 (2), Hela (3), HepG2 (4), Jurkat (5), K562 (6), L121O (7) and MCF-7 (8) cell lysate.)
Related Product Information for anti-ST13 antibody
Description: ST13 (suppression of tumorigenicity protein 13), also known as Hip (HSP70-interacting protein), is one of several co-chaperones that regulate activities of the HSP70 chaperone family. The homo-oligomeric protein Hip cooperates with HSP70 in protein folding by stabilizing the ADP-bound state of HSP70. Hip directly binds to the ATPase domain of HSP70 when it is converted to the ADP-bound state by proteins of the HSP40 family. By collaborating with other positive co-factors such as HSP40 and Hop, or competing with negative co-factors such as Bag1, Hip may facilitate the chaperone function of HSP70 in protein folding and repair, and in controlling the activity of regulatory proteins such as steroid receptors and various regulators of proliferation or apoptosis.
Function: One HIP oligomer binds the ATPase domains of at least two HSC70 molecules dependent on activation of the HSC70 ATPase by HSP40. Stabilizes the ADP state of HSC70 that has a high affinity for substrate protein. Through its own chaperone activity, it may contribute to the interaction of HSC70 with various target proteins (By similarity).
Subunit Structure: Homotetramer. Interacts with HSC70 as well as DNAJ homologs and HSP90 (By similarity). Interacts (via the C-terminus 303-319 AA) with GRK5.
Similarity: Belongs to the FAM10 family.
Function: One HIP oligomer binds the ATPase domains of at least two HSC70 molecules dependent on activation of the HSC70 ATPase by HSP40. Stabilizes the ADP state of HSC70 that has a high affinity for substrate protein. Through its own chaperone activity, it may contribute to the interaction of HSC70 with various target proteins (By similarity).
Subunit Structure: Homotetramer. Interacts with HSC70 as well as DNAJ homologs and HSP90 (By similarity). Interacts (via the C-terminus 303-319 AA) with GRK5.
Similarity: Belongs to the FAM10 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 48 kDa
Predicted: 42 kDa
Predicted: 42 kDa
NCBI Official Full Name
hsc70-interacting protein isoform 1
NCBI Official Synonym Full Names
ST13, Hsp70 interacting protein
NCBI Official Symbol
ST13
NCBI Official Synonym Symbols
HIP; HOP; P48; AAG2; SNC6; HSPABP; FAM10A1; FAM10A4; HSPABP1; PRO0786
NCBI Protein Information
hsc70-interacting protein
UniProt Protein Name
Hsc70-interacting protein
Protein Family
UniProt Gene Name
ST13
UniProt Synonym Gene Names
AAG2; FAM10A1; HIP; SNC6; Hip
NCBI Description
The protein encoded by this gene is an adaptor protein that mediates the association of the heat shock proteins HSP70 and HSP90. This protein has been shown to be involved in the assembly process of glucocorticoid receptor, which requires the assistance of multiple molecular chaperones. The expression of this gene is reported to be downregulated in colorectal carcinoma tissue suggesting that it is a candidate tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013]
Uniprot Description
One HIP oligomer binds the ATPase domains of at least two HSC70 molecules dependent on activation of the HSC70 ATPase by HSP40. Stabilizes the ADP state of HSC70 that has a high affinity for substrate protein. Through its own chaperone activity, it may contribute to the interaction of HSC70 with various target proteins ().
Research Articles on ST13
Similar Products
Product Notes
The ST13 st13 (Catalog #AAA9602709) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ST13 Antibody reacts with Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's ST13 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). ELISA: 1:10000 WB: 1:500-1:2000 IHC: 1:200-1:1000 ICC: 1:200-1:1000. Researchers should empirically determine the suitability of the ST13 st13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ST13, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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