Hip/ST13 recombinant protein

Hip/ST13, 1-369aa, Human, E Coli

Cat. Num. AAA203191

• Gene Names: ST13; HIP; HOP; P48; AAG2; SNC6; HSPABP; FAM10A1; FAM10A4; HSPABP1; PRO0786
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: Hip/ST13; 1-369aa; Human; E Coli; Hsc70-interacting protein; ST13; HOP; SNC6; FAM10A1; Hip/ST13 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 0.1M NaCl, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MDPRKVNELR AFVKMCKQDP SVLHTEEMRF LREWVESMGG KVPPATQKAK SEENTKEEKP DSKKVEEDLK ADEPSSEESD LEIDKEGVIE PDTDAPQEMG DENAEITEEM MDQANDKKVA AIEALNDGEL QKAIDLFTDA IKLNPRLAIL YAKRASVFVK LQKPNAAIRD CDRAIEINPD SAQPYKWRGK AHRLLGHWEE AAHDLALACK LDYDEDASAM LKEVQPRAQK IAEHRRKYER KREEREIKER IERVKKAREE HERAQREEEA RRQSGAQYGS FPGGFPGGMP GNFPGGMPGM GGGMPGMAGM PGLNEILSDP EVLAAMQDPE VMVAFQDVAQ NPANMSKYQS NPKVMNLISK LSAKFGGQA
• Sequence Length: 369

• Applicable Applications for Hip/ST13 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for Hip/ST13 recombinant protein

Hip (Hsc70-interacting protein), also known as ST13, is a co-chaperone to the major heat shock proteins, Hsp70 and Hsp90, and appears in early receptor complexes. Through mutual binding to both Hsp70 and Hsp90, Hip functions as an adaptor that can integrate Hsp70 and Hsp90 interactions. Also, Hip has been shown to be involved in the assembly process of glucocorticoid receptor, which requires the assistance of multiple molecular chaperones. Recombinant human Hip was expressed in E Coli and purified by using conventional chromatography

Product Categories/Family for Hip/ST13 recombinant protein

Heat Shock Proteins

References

Nelson GM., et al. (2004). Mol Endocrinol. 18 (7):1620-30; Johnson BD., et al. (1998). J Biol chem. 273 (6):3679-86;

NCBI and Uniprot Product Information

• NCBI GI #: 19923193
• NCBI GeneID: 6767
• NCBI Accession #: NP_003923
• NCBI GenBank Nucleotide #: NM_003932.4
• UniProt Accession #: P50502; O14999; Q2TU77
• Molecular Weight: 41.3 kDa (369aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: hsc70-interacting protein isoform 1
• NCBI Official Synonym Full Names: ST13, Hsp70 interacting protein
• NCBI Official Symbol: ST13
• NCBI Official Synonym Symbols: HIP; HOP; P48; AAG2; SNC6; HSPABP; FAM10A1; FAM10A4; HSPABP1; PRO0786
• NCBI Protein Information: hsc70-interacting protein
• UniProt Protein Name: Hsc70-interacting protein
• UniProt Gene Name: ST13
• UniProt Synonym Gene Names: AAG2; FAM10A1; HIP; SNC6; Hip

NCBI Description

The protein encoded by this gene is an adaptor protein that mediates the association of the heat shock proteins HSP70 and HSP90. This protein has been shown to be involved in the assembly process of glucocorticoid receptor, which requires the assistance of multiple molecular chaperones. The expression of this gene is reported to be downregulated in colorectal carcinoma tissue suggesting that it is a candidate tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013]

Uniprot Description

One HIP oligomer binds the ATPase domains of at least two HSC70 molecules dependent on activation of the HSC70 ATPase by HSP40. Stabilizes the ADP state of HSC70 that has a high affinity for substrate protein. Through its own chaperone activity, it may contribute to the interaction of HSC70 with various target proteins ().