Rabbit HSC70-Interacting Protein Polyclonal Antibody | anti-ST13 antibody

HSC70-Interacting Protein (HIP)

Cat. Num. AAA613398

• Gene Names: ST13; HIP; HOP; P48; AAG2; SNC6; HSPABP; FAM10A1; FAM10A4; HSPABP1; PRO0786; FLJ27260; MGC129952
• Reactivity: Bovine, Human, Mouse, Rat
• Applications: Western Blot
• Purity: Serum
• Synonyms: HSC70-Interacting Protein; Polyclonal Antibody; HSC70-Interacting Protein (HIP); Anti -HSC70-Interacting Protein (HIP); anti-ST13 antibody

• Host: Rabbit
• Reactivity: Bovine, Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects a ~58kD protein, corresponding to the apparent molecular mass of Hip on SDS-PAGE immunoblots, in samples from human, mouse, rat and bovine. The antibody detects both recombinant rat Hip as well as native Hip protein in tissue extracts. Whilst reactivity with human and bovine Hip protein is detected, this reactivity is much weaker than that observed with samples from rat or mouse origin.
• Purity/Purification: Serum
• Form/Format: Serum

• Applicable Applications for anti-ST13 antibody: Western Blot (WB)
• Application Notes: Western Blot (Colorimetric): 1:5,000 (mouse, rat), Western Blot (Colorimetric): 1:1,000 (human, bovine), Optimal dilutions to be determined by researcher., Positive Controls, Recombinant Rat Hip Protein
• Immunogen: Recombinant rat Hip histidine 6 fusion protein.
• Preparation and Storage: -20 degree C

Western Blot (WB)

(Western blot analysis: Lane 1: MW marker, Lane 2: recombinant rat Hip, Lane 3: A431 cell lysate, Lane 4: mouse brain tissue extract, Lane 5: rat brain tissue extract, probed with MBS613398.)

Related Product Information for anti-ST13 antibody

Hsc70-interacting protein (Hip) is a cytosolic protein that exists as a homo-oligomer and participates in the regulation of the heat shock protein Hsc70 in eukaryotic cells (1). Hip interacts with the ATPase domain of Hsc70 molecules through its tetratricopeptide repeats and flanking charged alpha-helices (2). Hip and BAG-1 proteins have been shown to compete in binding to the ATPase domain (4). This binding reaction is dependent on Hsp40 in the presence of ATP and becomes Hsp40-independent if Hsc70 is preincubated with ADP (2). Upon binding to Hsc70, Hip stabilizes the ADP-bound state of Hsc70, a conformation that has a high affinity for unfolded substrate proteins. Hip by itself binds unfolded polypeptides, suggesting that it may possess chaperone activity (2,3). Together with Hsp40 and BAG-1, Hip may regulate the chaperone activity of Hsc70 by stabilizing the chaperone-substrate complex.

Product Categories/Family for anti-ST13 antibody

Antibodies; Abs to Heat Shock Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 19923193
• NCBI GeneID: 6767
• NCBI Accession #: NP_003923.2
• NCBI GenBank Nucleotide #: NM_003932.3
• UniProt Accession #: P50502; O14999; Q0IJ56; Q1XBU6; Q1XBU7; Q2TU77; Q3KNR6; Q9P1I4
• Molecular Weight: 41,332 Da
• NCBI Official Full Name: hsc70-interacting protein
• NCBI Official Synonym Full Names: suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein)
• NCBI Official Symbol: ST13
• NCBI Official Synonym Symbols: HIP; HOP; P48; AAG2; SNC6; HSPABP; FAM10A1; FAM10A4; HSPABP1; PRO0786; FLJ27260; MGC129952
• NCBI Protein Information: hsc70-interacting protein; OTTHUMP00000199376; Hsp70-interacting protein; aging-associated protein 2; putative tumor suppressor ST13; renal carcinoma antigen NY-REN-33; heat shock 70kD protein binding protein; suppression of tumorigenicity 13 protein; progesterone receptor-associated p48 protein
• UniProt Protein Name: Hsc70-interacting protein
• Protein Family: Hsc70-interacting protein
• UniProt Gene Name: ST13
• UniProt Synonym Gene Names: FAM10A1; HIP; SNC6
• UniProt Entry Name: F10A1_HUMAN

NCBI Description

The protein encoded by this gene is an adaptor protein that mediates the association of the heat shock proteins HSP70 and HSP90. This protein has been shown to be involved in the assembly process of glucocorticoid receptor, which requires the assistance of multiple molecular chaperones. The expression of this gene is reported to be downregulated in colorectal carcinoma tissue suggesting that is a candidate tumor suppressor gene. [provided by RefSeq]

Uniprot Description

HIP: One HIP oligomer binds the ATPase domains of at least two HSC70 molecules dependent on activation of the HSC70 ATPase by HSP40. Stabilizes the ADP state of HSC70 that has a high affinity for substrate protein. Through its own chaperone activity, it may contribute to the interaction of HSC70 with various target proteins. Homotetramer. Interacts with HSC70 as well as DNAJ homologs and HSP90. Interacts (via the C-terminus 303- 319 AA) with GRK5. Belongs to the FAM10 family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: protein complex; cytoplasm; cytosol

Molecular Function: protein binding, bridging; identical protein binding; protein domain specific binding; protein binding; chaperone binding; unfolded protein binding; dATP binding; protein complex binding; Hsp70 protein binding

Biological Process: protein folding; protein homooligomerization