Immunocytochemistry (ICC)
(Immunocytochemistry Analysis:|Confocal immunofluorescence analysis of A431 cells using anti-SRF (Red). Actin filaments have been labeled with Alexa Fluor 488-Phalloidin (Green). Nucleus is stained with DAPI (Blue).)
Mouse anti-Human, Mouse SRF Monoclonal Antibody
SRF (Serum Response Factor, c-fos Serum Response Element-binding Transcription Factor, MADS Family Member)
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
SRF; Monoclonal Antibody; SRF (Serum Response Factor; c-fos Serum Response Element-binding Transcription Factor; MADS Family Member); Anti -SRF (Serum Response Factor; anti-SRF antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1k
Clone Number
9H277
Specificity
Recognizes human SRF. Species Crossreactivity: mouse.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in 0.1M Tris-Glycine, pH7.4, 150mM sodium chloride, 0.05% sodium azide.
Applicable Applications for anti-SRF antibody
Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
Suitable for use in Western Blot and Immunocytochemistry.
Dilution: Western Blot: 0.5ug/ml
Immunocytochemistry: 1:500
Dilution: Western Blot: 0.5ug/ml
Immunocytochemistry: 1:500
Immunogen
Synthetic human SRF peptide, aa406-508. Cellular Localization: Nucleus.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunocytochemistry (ICC)
(Immunocytochemistry Analysis:|Confocal immunofluorescence analysis of A431 cells using anti-SRF (Red). Actin filaments have been labeled with Alexa Fluor 488-Phalloidin (Green). Nucleus is stained with DAPI (Blue).)
Immunocytochemistry (ICC)
(Immunocytochemistry Analysis:|Confocal immunofluorescence analysis of A431 cells using anti-SRF (Red). Actin filaments have been labeled with Alexa Fluor 488-Phalloidin (Green). Nucleus is stained with DAPI (Blue).)
Western Blot (WB)
(Western Blot Analysis:|HeLa nuclear cell lysates were resolved by electrophoresis, transferred to PVDF and probed with anti-SRF (0.5 ug/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system .)
Western Blot (WB)
(Western Blot Analysis:|HeLa nuclear cell lysates were resolved by electrophoresis, transferred to PVDF and probed with anti-SRF (0.5 ug/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system .)
Related Product Information for anti-SRF antibody
Serum response factor (SRF) is a nuclear transcription factor that binds to the serum response element (SRE), and is essential for embryonic development of the mesoderm, as well as for the maintenance of adult muscle cells and neurons. As this protein is involved in the downstream regulation of many immediate-early genes, it participates in a variety of cell functions, including cell cycle, apoptosis, cell growth, and cell differentiation. SRF is most commonly activated by serum, LPA, LPS, TPA, growth factors, including TNFalpha, as well as antioxidants and UV light.
Product Categories/Family for anti-SRF antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The SRF (Catalog #AAA632020) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SRF (Serum Response Factor, c-fos Serum Response Element-binding Transcription Factor, MADS Family Member) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SRF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC). Suitable for use in Western Blot and Immunocytochemistry. Dilution: Western Blot: 0.5ug/ml Immunocytochemistry: 1:500. Researchers should empirically determine the suitability of the SRF for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SRF, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
