Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) Recombinant Protein | PPARgC1a recombinant protein

Recombinant Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a)

Cat. Num. AAA2010580

• Gene Names: Ppargc1a; Pgc1; PGC-1; Pgco1; PGC-1v; Gm11133; Ppargc1; Pgc-1alpha; A830037N07Rik; ENSMUSG00000079510
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a); Recombinant Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a); PPARgC1a recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- CSRS PYSRSRSRSP GSRSSSRSCY YYESSHYRHR THRNSPLYVR SRSRSPYSRR PRYDSYEAYE HERLKRDEYR KEHEKRESER AKQRERQKQK AIEERRVIYV GKIRPDTTRT ELRDRFEVFG EIEECTVNLR DDGDSYGFIT YRYTCDAFAA LENGYTLRRS NETDFELYFC GRKQFFKSNY ADLDTN
• Sequence Length: 797

• Applicable Applications for PPARgC1a recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Cys577~Asn766 (Accession # O70343) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6679433
• NCBI GeneID: 19017
• NCBI Accession #: NP_032930.1
• NCBI GenBank Nucleotide #: NM_008904.2
• UniProt Accession #: O70343; Q3UP72; L0AM20; L0AN96; L0APB0
• Molecular Weight: 24.7kDa
• NCBI Official Full Name: peroxisome proliferator-activated receptor gamma coactivator 1-alpha
• NCBI Official Synonym Full Names: peroxisome proliferative activated receptor, gamma, coactivator 1 alpha
• NCBI Official Symbol: Ppargc1a
• NCBI Official Synonym Symbols: Pgc1; PGC-1; Pgco1; PGC-1v; Gm11133; Ppargc1; Pgc-1alpha; A830037N07Rik; ENSMUSG00000079510
• NCBI Protein Information: peroxisome proliferator-activated receptor gamma coactivator 1-alpha; PGC-1-alpha; Pgc-1alphaa; PPARGC-1-alpha; PPAR Gamma Coactivator-1; PPAR-gamma coactivator 1-alpha; PPAR gamma coactivator variant form; peroxisome proliferator-activated receptor gamma coactivator-1 alpha
• UniProt Protein Name: Peroxisome proliferator-activated receptor gamma coactivator 1-alpha
• Protein Family: Peroxisome proliferator-activated receptor gamma coactivator
• UniProt Gene Name: Ppargc1a
• UniProt Synonym Gene Names: Pgc1; Pgc1a; Ppargc1; PGC-1-alpha; PPAR-gamma coactivator 1-alpha
• UniProt Entry Name: PRGC1_MOUSE

Uniprot Description

PGC-1 alpha: a transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor genes. Interacts with, and regulates the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). Regulates many genes involved in energy metabolism. Provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. May be involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. Its transcription is repressed by ZNF746 which binds to 'insulin response sequences' in its promoter. Heavily acetylated by GCN5 and biologically inactive under conditions of high nutrients. Deacetylated by SIRT1 in low nutrients/high NAD conditions.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Cellular Component: cytosol; nucleus

Molecular Function: ligand-dependent nuclear receptor binding; protein binding; nucleic acid binding; ligand-dependent nuclear receptor transcription coactivator activity; DNA binding; sequence-specific DNA binding; RNA binding; chromatin DNA binding; ubiquitin protein ligase binding; transcription coactivator activity; nucleotide binding; chromatin binding; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; mitochondrion organization and biogenesis; response to dietary excess; response to muscle activity; transcription, DNA-dependent; positive regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-dependent; positive regulation of cellular metabolic process; rhythmic process; negative regulation of smooth muscle cell migration; regulation of circadian rhythm; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; positive regulation of fatty acid oxidation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; circadian regulation of gene expression; negative regulation of neuron apoptosis; negative regulation of glycolysis