DAPK1 / DAP Kinase 1 Active Protein | DAPK1 active protein

Recombinant Human DAPK1 / DAP Kinase 1 Protein (aa 1-363, His & GST tag)

Cat. Num. AAA2545044

• Gene Names: DAPK1; DAPK
• Purity: (78.5 + 12.2%) as determined by SDS-PAGE
• Synonyms: DAPK1 / DAP Kinase 1; Recombinant Human DAPK1 / DAP Kinase 1 Protein (aa 1-363; His & GST tag); RP11-104M22.1; MGC126323; MGC126324; DAPK1 active protein

• Purity/Purification: (78.5 + 12.2%) as determined by SDS-PAGE
• Form/Format: Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly
• Concentration: 0.3 mg/mL measured by UV-Vis (varies by lot)
• Sequence Length: 1430

• Source: Human
• Expression Host: Baculovirus-insect cells
• Protein Construction: A DNA sequence encoding the N-terminal segment of human DAPK1 (P53355-1) (Met 1-Leu 363) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Bio-Activity: The specific was determined to be 20 nmo/min/mg using synthetic R11-S6-Peptid (R11-IAKRRRLSSLRASTSKSESSQK) as substrate.
• Predicted N-terminal: Met
• Moleclar Mass: The recombinant human DAPK1 (aa 1-363)/GST chimera consists of 600 amino acids and has a calculated molecular mass of 69.4 kDa. It migrates as an approximately 64 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method
• Component: Total Volume is 67 uL
• Preparation and Storage: Storage: Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliqouted and be used as soon as possible.; Avoid repeated freeze-thaw cycles.; Stability: Samples are stable for up to twelve months from date of receipt at -70 °.C

SDS-PAGE

Related Product Information for DAPK1 active protein

Death-associated protein kinase 1, also known as DAP kinase 1, DAPK1 and DAPK, is a cytoplasm protein which belongs to the protein kinase superfamily, CAMK Ser / Thr protein kinase family and DAP kinase subfamily. DAPK1 contains ten ANK repeats, one death domain and one protein kinase domain. DAPK1 is a calcium / calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis. DAPK1 gene is a candidate tumor suppressor (TSG) and the abnormal methylation of DAPK1 gene has been found in many carcinomas. DAPK1 over-expression can induce cell apoptosis and inhibit tumor cell metastasis. DAPK1 gene over-expression could suppress PGCl3 cells malignant phenotype , inhibit PGCl3 cells growth, invasive, migration and adhesion ability, upregulate p53 gene and downregulate bcl-2 gene. Loss of activity of death-associated protein kinase 1 (DAPK1) may be an independent factor affecting survival of non-small cell lung cancer patients. DAPK1 promoter methylation might play a significant role in the progression of chronic myeloid leukemia (CML).

NCBI and Uniprot Product Information

• NCBI GI #: 570359575
• NCBI GeneID: 1612
• NCBI Accession #: NP_001275658.1
• NCBI GenBank Nucleotide #: NM_001288729.1
• UniProt Accession #: P53355; Q14CQ7; Q1W5W0; Q68CP8; Q6ZRZ3; Q9BTL8; B7ZLD2; B7ZLE7
• NCBI Official Full Name: death-associated protein kinase 1
• NCBI Official Synonym Full Names: death associated protein kinase 1
• NCBI Official Symbol: DAPK1
• NCBI Official Synonym Symbols: DAPK
• NCBI Protein Information: death-associated protein kinase 1
• UniProt Protein Name: Death-associated protein kinase 1
• Protein Family: Death-associated protein kinase
• UniProt Gene Name: DAPK1
• UniProt Synonym Gene Names: DAPK; DAP kinase 1

NCBI Description

Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Uniprot Description

Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca2+ influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition.