Western Blot (WB)
(Western blot detection of Keratin 18 in Hela,K562 cell lysates using Keratin 18 Rabbit pAb(1:1000 diluted).Predicted band size:48KDa.Observed band size:48KDa.)
Rabbit Cytokeratin 18 Polyclonal Antibody | anti-Krt18 antibody
Cytokeratin 18 Rabbit pAb
Gene Names
Krt18; K18; CK18; Krt1-18
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
Cytokeratin 18; Polyclonal Antibody; Cytokeratin 18 Rabbit pAb; keratin 18; K18; CK18; Krt1-18; anti-Krt18 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Expressed in endoderm, intestinal epithelial cells and in most extraembryonic tissues.
Purity/Purification
Affinity purified
Form/Format
Supplied in 50nM Tris-Glycine(pH7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Applicable Applications for anti-Krt18 antibody
Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:1000
IHC: 1:20-1:500
ICC/IF: 1:20
FC/FACS: 1:20
IHC: 1:20-1:500
ICC/IF: 1:20
FC/FACS: 1:20
Immunogen
Recombinant protein of human Cytokeratin 18
Conjugation
Unconjugated
Molecular Function
When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Involved in the uptake of thrombin-antithrombin complexes by hepatic cells. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Modification
Unmodified
Cell Position
Cytoplasm, Intermediate filament, Keratin, Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot detection of Keratin 18 in Hela,K562 cell lysates using Keratin 18 Rabbit pAb(1:1000 diluted).Predicted band size:48KDa.Observed band size:48KDa.)
Western Blot (WB)
(Western blot detection of Keratin 18 in Hela,K562 cell lysates using Keratin 18 Rabbit pAb(1:1000 diluted).Predicted band size:48KDa.Observed band size:48KDa.)
Immunocytochemistry (ICC)
(Immunocytochemistry of Cytokeratin 18(green) in Hela cells using Cytokeratin 18 Rabbit pAb at dilution 1/50, and DAPI(blue))
Immunocytochemistry (ICC)
(Immunocytochemistry of Cytokeratin 18(green) in Hela cells using Cytokeratin 18 Rabbit pAb at dilution 1/50, and DAPI(blue))
Immunohistochemistry (IHC)
(Immunohistochemistry of Cytokeratin 18 in paraffin-embedded Human Cholangiocarcinoma using Cytokeratin 18 Rabbit pAb at dilution 1/20)
Immunohistochemistry (IHC)
(Immunohistochemistry of Cytokeratin 18 in paraffin-embedded Human Cholangiocarcinoma using Cytokeratin 18 Rabbit pAb at dilution 1/20)
Product Categories/Family for anti-Krt18 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47538 MW
NCBI Official Full Name
keratin, type I cytoskeletal 18
NCBI Official Synonym Full Names
keratin 18
NCBI Official Symbol
Krt18
NCBI Official Synonym Symbols
K18; CK18; Krt1-18
NCBI Protein Information
keratin, type I cytoskeletal 18
UniProt Protein Name
Keratin, type I cytoskeletal 18
Protein Family
UniProt Gene Name
Krt18
UniProt Synonym Gene Names
Kerd; Krt1-18; Keratin D; CK-18; K18
Uniprot Description
When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (). Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Research Articles on Krt18
Similar Products
Product Notes
The Krt18 krt18 (Catalog #AAA4755571) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cytokeratin 18 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cytokeratin 18 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:1000 IHC: 1:20-1:500 ICC/IF: 1:20 FC/FACS: 1:20. Researchers should empirically determine the suitability of the Krt18 krt18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin 18, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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