Mouse anti-Human SHMT / SHMT1 Monoclonal Antibody | anti-SHMT antibody

Mouse Monoclonal [clone 4F9] (IgG1,k) to Human SHMT / SHMT1

Cat. Num. AAA246261

• Gene Names: SHMT1; SHMT; CSHMT
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: SHMT / SHMT1; Monoclonal Antibody; Mouse Monoclonal [clone 4F9] (IgG1; k) to Human SHMT / SHMT1; Anti-SHMT / SHMT1 Antibody (clone 4F9) IHC-plus; SHMT1; 14 kDa protein; CSHMT; Serine methylase; SHMT; Human SHMT; anti-SHMT antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 4F9
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Concentration: 0.46 mg/ml (varies by lot)
• Sequence Length: 483

• Applicable Applications for anti-SHMT antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB; Usage: Western Blot (Cell lysate) - positive control HeLa. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Sandwich ELISA (Recombinant protein).
• Target Species: Human
• Immunogen Description: SHMT1 (NP_004160, 374 a.a. ~ 483 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Type: Recombinant protein
• Immunogen: SHMT / SHMT1 antibody was raised against sHMT1 (NP_004160, 374 a.a. ~ 483 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-SHMT1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-SHMT1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-SHMT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of SHMT1 expression in transfected 293T cell line by SHMT1 monoclonal antibody clone 4F9.)

Western Blot (WB)

(SHMT1 monoclonal antibody clone 4F9 Western blot of SHMT1 expression in HeLa.)

ELISA (EIA)

(Detection limit for recombinant GST tagged SHMT1 is approximately 1 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 22547186
• NCBI GeneID: 6470
• NCBI Accession #: NP_004160.3
• NCBI GenBank Nucleotide #: NM_004169.4
• UniProt Accession #: P34896; Q96HY0; Q9UMD1; Q9UMD2; B4DPM9; D3DXD0
• Molecular Weight: 37,672 Da
• NCBI Official Full Name: serine hydroxymethyltransferase, cytosolic isoform 1
• NCBI Official Synonym Full Names: serine hydroxymethyltransferase 1 (soluble)
• NCBI Official Symbol: SHMT1
• NCBI Official Synonym Symbols: SHMT; CSHMT
• NCBI Protein Information: serine hydroxymethyltransferase, cytosolic; serine methylase; glycine hydroxymethyltransferase; cytoplasmic serine hydroxymethyltransferase
• UniProt Protein Name: Serine hydroxymethyltransferase, cytosolic
• UniProt Gene Name: SHMT1
• UniProt Synonym Gene Names: SHMT
• UniProt Entry Name: GLYC_HUMAN

NCBI Description

This gene encodes the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one-carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. A pseudogene of this gene is located on the short arm of chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

SHMT1: Interconversion of serine and glycine. Belongs to the SHMT family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - glycine, serine and threonine; Mitochondrial; Cofactor and Vitamin Metabolism - one carbon pool by folate; Methyltransferase; EC 2.1.2.1; Lyase; Other Amino Acids Metabolism - cyanoamino acid; Energy Metabolism - methane

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus; cytosol

Molecular Function: L-allo-threonine aldolase activity; amino acid binding; protein homodimerization activity; glycine hydroxymethyltransferase activity; pyridoxal phosphate binding

Biological Process: purine base biosynthetic process; vitamin metabolic process; folic acid metabolic process; L-serine catabolic process; glycine biosynthetic process from serine; protein tetramerization; water-soluble vitamin metabolic process; carnitine biosynthetic process; protein homotetramerization