Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Zinc transporter 4 (SLC30A4) Standard Curve.)
Mouse Zinc Transporter 4 (SLC30A4) ELISA Kit | ZNT4 elisa kit
Mouse Zinc Transporter 4 (SLC30A4) ELISA Kit
Gene Names
Slc30a4; lm; Znt4; znT-4
Reactivity
Mouse
Synonyms
Zinc Transporter 4 (SLC30A4); Mouse Zinc Transporter 4 (SLC30A4) ELISA Kit; SLC30A4; ZNT4 elisa kit
Reactivity
Mouse
Specificity
Mouse Zinc transporter 4 (SLC30A4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SLC30A4. No significant cross-reactivity or interference between Mouse SLC30A4 and analogues was observed.
Sequence Length
381
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Zinc transporter 4 (SLC30A4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Zinc transporter 4 (SLC30A4) Standard Curve.)
Related Product Information for ZNT4 elisa kit
Description: This Mouse Zinc transporter 4 (SLC30A4) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC30A4 in samples. An antibody specific for SLC30A4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC30A4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC30A4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC30A4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Zinc is the second most abundant trace metal in the human body. It is an essential element, serving both a structural role, as in the formation of zinc fingers in DNA-binding proteins, and a catalytic role in metalloenzymes, such as pancreatic carboxypeptidases, alkaline phosphatases, various dehydrogenases, and superoxide dismutases. SLC30A4, or ZNT4, belongs to the ZNT family of zinc transporters. ZNTs are involved in transporting zinc out of the cytoplasm and have similar structures, consisting of 6 transmembrane domains and a histidine-rich cytoplasmic loop. The gene encodes a 430-amino acid protein that is homologous to Znt2 (SLC30A2) and Znt3 (SLC30A3). Znt4 was abundantly expressed in mammary epithelia and brain.
Background: Zinc is the second most abundant trace metal in the human body. It is an essential element, serving both a structural role, as in the formation of zinc fingers in DNA-binding proteins, and a catalytic role in metalloenzymes, such as pancreatic carboxypeptidases, alkaline phosphatases, various dehydrogenases, and superoxide dismutases. SLC30A4, or ZNT4, belongs to the ZNT family of zinc transporters. ZNTs are involved in transporting zinc out of the cytoplasm and have similar structures, consisting of 6 transmembrane domains and a histidine-rich cytoplasmic loop. The gene encodes a 430-amino acid protein that is homologous to Znt2 (SLC30A2) and Znt3 (SLC30A3). Znt4 was abundantly expressed in mammary epithelia and brain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
zinc transporter 4 isoform b
NCBI Official Synonym Full Names
solute carrier family 30 (zinc transporter), member 4
NCBI Official Symbol
Slc30a4
NCBI Official Synonym Symbols
lm; Znt4; znT-4
NCBI Protein Information
zinc transporter 4
UniProt Protein Name
Zinc transporter 4
UniProt Gene Name
Slc30a4
UniProt Synonym Gene Names
Lm; Znt4; ZnT-4
Uniprot Description
Probably involved in zinc transport out of the cytoplasm, may be by sequestration into an intracellular compartment.
Research Articles on ZNT4
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Product Notes
The Mouse ZNT4 slc30a4 (Catalog #AAA9717034) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717034 ELISA Kit recognizes Mouse ZNT4. It is sometimes possible for the material contained within the vial of "Zinc Transporter 4 (SLC30A4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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