Rabbit anti-Human SF3B3 Polyclonal Antibody | anti-SF3B3 antibody

SF3B3, CT (SF3B3, KIAA0017, SAP130, Splicing factor 3B subunit 3, Pre-mRNA-splicing factor SF3b 130kD subunit, STAF130, Spliceosome-associated protein 130) (Azide free) (HRP)

Cat. Num. AAA6346552

• Gene Names: SF3B3; RSE1; SAP130; SF3b130; STAF130
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SF3B3; Polyclonal Antibody; CT (SF3B3; KIAA0017; SAP130; Splicing factor 3B subunit 3; Pre-mRNA-splicing factor SF3b 130kD subunit; STAF130; Spliceosome-associated protein 130) (Azide free) (HRP); Spliceosome-associated protein 130; anti-SF3B3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SF3B3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SF3B3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1053-1082 amino acids from the C-terminal region of human SF3B3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SF3B3 Antibody (C-term) western blot analysis in MDA-MB453,Jurkat cell line lysates (35ug/lane).This demonstrates the SF3B3 antibody detected the SF3B3 protein (arrow).)

Related Product Information for anti-SF3B3 antibody

This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.

Product Categories/Family for anti-SF3B3 antibody

Antibodies; Splicing Factors

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Will, C.L., et al. RNA 10(6):929-941(2004). Cavusoglu, N., et al. Proteomics 3(2):217-223(2003). Will, C.L., et al. EMBO J. 21(18):4978-4988(2002). Martinez, E., et al. Mol. Cell. Biol. 21(20):6782-6795(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 54112121
• NCBI GeneID: 23450
• NCBI Accession #: NP_036558.3
• NCBI GenBank Nucleotide #: NM_012426.4
• UniProt Accession #: Q15393; Q6NTI8; Q96GC0; Q9BPY2; Q9UFX7; Q9UJ29
• Molecular Weight: 135,577 Da
• NCBI Official Full Name: splicing factor 3B subunit 3
• NCBI Official Synonym Full Names: splicing factor 3b, subunit 3, 130kDa
• NCBI Official Symbol: SF3B3
• NCBI Official Synonym Symbols: RSE1; SAP130; SF3b130; STAF130
• NCBI Protein Information: splicing factor 3B subunit 3; SAP 130; spliceosome-associated protein 130; pre-mRNA-splicing factor SF3b 130 kDa subunit; pre-mRNA splicing factor SF3b, 130 kDa subunit
• UniProt Protein Name: Splicing factor 3B subunit 3
• Protein Family: Splicing factor
• UniProt Gene Name: SF3B3
• UniProt Synonym Gene Names: KIAA0017; SAP130; SF3b130; SAP 130
• UniProt Entry Name: SF3B3_HUMAN

NCBI Description

This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. [provided by RefSeq, Jul 2008]

Uniprot Description

SF3B3: Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. Belongs to the RSE1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; RNA splicing; Spliceosome

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: small nuclear ribonucleoprotein complex; nucleoplasm; spliceosome; U12-dependent spliceosome

Molecular Function: protein binding; RNA binding

Biological Process: RNA splicing, via transesterification reactions; nuclear mRNA splicing, via spliceosome; RNA splicing; protein complex assembly; gene expression; mRNA processing