Mouse SCD1 Monoclonal Antibody | anti-SCD1 antibody

Anti-SCD1 Antibody

Cat. Num. AAA415297

• Gene Names: Scd1; ab; Scd; Scd-1; AA589638; AI265570
• Applications: Western Blot, ELISA, Immunoprecipitation, Immunohistochemistry
• Synonyms: SCD1; Monoclonal Antibody; Anti-SCD1 Antibody; anti-SCD1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: CD.E10
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 355

• Applicable Applications for anti-SCD1 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Western blot, ELISA, IP, and IHC in obesity research. Stearoyl-CoA desaturase (SCD) is a regulatory enzyme in lipogenesis. The SCD1 gene is tightly regulated by signals such as insulin, leptin, carbohydrate, fatty acids and temperature. The enzyme (mouse 355aa, rat 358aa; ~37 KDa) is constitutively expressed in adipose tissue and is induced in liver in response to fat free, high carbohydrate diet. The SCD-1 expression is higher in female than male mouse. The repression of RNA levels and enzyme activity of hepatic SCD-1 by Leptin has been shown to significantly reduce the triglyceride storage, VLDL production and therefore an increase in fatty acid oxidation. Inhibition of SCD-1 could be of benefit for treatment of obesity, hepatic steatosis and other metabolic disorders.
• Storage Buffer: PBS, pH 7.4 with 0.05% sodium azide.
• Immunogen: Full length recombinant protein.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

NCBI and Uniprot Product Information

• NCBI GI #: 71059993
• NCBI GeneID: 20249
• UniProt Accession #: Q547C4; P13516
• Molecular Weight: 41,046 Da
• NCBI Official Full Name: Scd1
• NCBI Official Synonym Full Names: stearoyl-Coenzyme A desaturase 1
• NCBI Official Symbol: Scd1
• NCBI Official Synonym Symbols: ab; Scd; Scd-1; AA589638; AI265570
• NCBI Protein Information: acyl-CoA desaturase 1; asebia; delta(9)-desaturase 1; delta-9 desaturase 1; fatty acid desaturase 1; stearoyl-CoA desaturase 1
• UniProt Protein Name: MCG131749
• Protein Family: Scytalone dehydratase
• UniProt Gene Name: Scd1
• UniProt Entry Name: Q547C4_MOUSE

Uniprot Description

SCD: Terminal component of the liver microsomal stearyl-CoA desaturase system, that utilizes O(2) and electrons from reduced cytochrome b5 to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA. Belongs to the fatty acid desaturase family.

Protein type: Lipid Metabolism - unsaturated fatty acid biosynthesis; Endoplasmic reticulum; Oxidoreductase; Membrane protein, integral; EC 1.14.19.1; Membrane protein, multi-pass

Cellular Component: membrane; intracellular membrane-bound organelle; endoplasmic reticulum; integral to membrane; integral to endoplasmic reticulum membrane

Molecular Function: iron ion binding; oxidoreductase activity; oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water; stearoyl-CoA 9-desaturase activity

Biological Process: defense response to Gram-positive bacterium; white fat cell differentiation; brown fat cell differentiation; lipid metabolic process; fatty acid metabolic process; fatty acid biosynthetic process