Acid Phosphatase 1 (ACP1) Recombinant Protein | ACP1 recombinant protein

Recombinant Acid Phosphatase 1 (ACP1)

Cat. Num. AAA2011646

• Gene Names: ACP1; HAAP
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Acid Phosphatase 1 (ACP1); Recombinant Acid Phosphatase 1 (ACP1); ACP1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGS-MAEQATKSVL FVCLGNICRS PIAEAVFRKL VTDQNISENW RVDSAATSGY EIGNPPDYRG QSCMKRHGIP MSHVARQITK EDFATFDYIL CMDESNLRDL NRKSNQVKTC KAKIELLGSY DPQKQLIIED PYYGNDSDFE TVYQQCVRCC RAFLEKAH
• Sequence Length: 158

• Applicable Applications for ACP1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Met1~His158 (Accession # P24666) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4757714
• NCBI GeneID: 52
• NCBI Accession #: NP_004291.1
• NCBI GenBank Nucleotide #: NM_004300.3
• UniProt Accession #: P24666; P24667; Q16035; Q16036; Q16725; Q3KQX8; Q53RU0; A8K1L9
• Molecular Weight: 19.3kDa
• NCBI Official Full Name: low molecular weight phosphotyrosine protein phosphatase isoform c
• NCBI Official Synonym Full Names: acid phosphatase 1, soluble
• NCBI Official Symbol: ACP1
• NCBI Official Synonym Symbols: HAAP
• NCBI Protein Information: low molecular weight phosphotyrosine protein phosphatase; LMW-PTP; LMW-PTPase; adipocyte acid phosphatase; red cell acid phosphatase 1; protein tyrosine phosphatase; acid phosphatase of erythrocyte; cytoplasmic phosphotyrosyl protein phosphatase; low molecular weight cytosolic acid phosphatase
• UniProt Protein Name: Low molecular weight phosphotyrosine protein phosphatase
• Protein Family: Adult cuticle protein
• UniProt Gene Name: ACP1
• UniProt Synonym Gene Names: LMW-PTP; LMW-PTPase
• UniProt Entry Name: PPAC_HUMAN

NCBI Description

The product of this gene belongs to the phosphotyrosine protein phosphatase family of proteins. It functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate. This enzyme also hydrolyzes orthophosphoric monoesters to alcohol and orthophosphate. This gene is genetically polymorphic, and three common alleles segregating at the corresponding locus give rise to six phenotypes. Each allele appears to encode at least two electrophoretically different isozymes, Bf and Bs, which are produced in allele-specific ratios. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

ACP1: a phosphotyrosine protein phosphatase. Functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate. Hydrolyzes orthophosphoric monoesters to alcohol and orthophosphate. Genetically polymorphic with three common alleles segregating at the corresponding locus give rise to six phenotypes. Each allele appears to encode at least two electrophoretically different isozymes, Bf and Bs, which are produced in allele-specific ratios. Three transcript variants encoding distinct isoforms have been identified for this gene.

Protein type: Phosphatase (non-protein); EC 3.1.3.48; EC 3.1.3.2; Motility/polarity/chemotaxis; Cofactor and Vitamin Metabolism - riboflavin; Protein phosphatase, tyrosine (non-receptor)

Chromosomal Location of Human Ortholog: 2p25

Cellular Component: internal side of plasma membrane; cytoplasm

Molecular Function: acid phosphatase activity; protein binding; non-membrane spanning protein tyrosine phosphatase activity