SARS-CoV/SARS-CoV-2 Nucleocapsid Monoclonal Antibody | anti-NP-CoV antibody

SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody, Chimeric MAb

Cat. Num. AAA8119467

• Reactivity: SARS
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
• Purity: Protein A
• Synonyms: SARS-CoV/SARS-CoV-2 Nucleocapsid; Monoclonal Antibody; SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody; Chimeric MAb; Anti-coronavirus NP Antibody; Anti-coronavirus Nucleocapsid Antibody; Anti-coronavirus Nucleoprotein Antibody; Anti-cov np Antibody; Anti-ncov NP Antibody; Anti-novel coronavirus NP Antibody; Anti-novel coronavirus Nucleocapsid Antibody; Anti-novel coronavirus Nucleoprotein Antibody; Anti-NP Antibody; Anti-Nucleocapsid Antibody; Anti-Nucleoprotein Antibody; anti-NP-CoV antibody

• Host: Chimeric Rabbit/Human
• Reactivity: SARS
• Clonality: Monoclonal
• Isotype: Rabbit (variable region)/Human (kappa/IgG1 constant) chimeric antibody
• Clone Number: 4
• Specificity: SARS-CoV Nucleocapsid Protein
• Purity/Purification: Protein A
• Form/Format: Liquid. 0.2um filtered solution in PBS

• Applicable Applications for anti-NP-CoV antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:2000-1:5000; ELISA: 1:5000-1:10,000; IHC-P: 1:20-1:100; ICC/IF: 1:20-1:100; FC/FACS/FCM: 1:25-1:100; Antibody's applications have not been validated with corresponding viruses.
• Immunogen: Recombinant SARS-CoV Nucleoprotein/NP Protein
• Conjugation: Unconjugated
• Cross-reactivity: Has cross-reactivity in ELISA with; SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein ; No cross-reactivity in ELISA with; MERS-CoV Nucleoprotein protein ; HCoV-229E Nucleoprotein protein ; HCoV-NL63 Nucleoprotein protein ; HCoV-HKU1(isolate N5) Nucleoprotein protein ; HCoV-OC43 Nucleoprotein
• Preparation: It is a chimeric monoclonal antibody combining the constant domains of the human IgG1 molecule with rabbit variable regions, and the variable region was from rabbit monoclonal antibody. The chimeric monoclonal antibody was produced using recombinant antibody technology.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.; This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of SARS-COV-2 Nucleocapsid overexpressed HEK293 Cells were stained with purified anti-SARS-COV-2 Nucleocapsid Chimeric MAb, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

Western Blot (WB)

(Anti-SARS-CoV / SARS-CoV-2 Nucleocapsid Chimeric monoclonal antibody at 1:2000 dilution. Lane A: SARS-COV-2 Nucleocapsid overexpressed HEK293 Whole Cell Lysate Lane B: HEK293 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Human IgG Fc/HRP at 1/1000 dilution Developed using the ECL technique. Performed under reducing conditions.)

Immunofluorescence (IF)

(Immunofluorescence analysis of SARS-COV-2 Nucleocapsid overexpressed HEK293 Cells were stained with purified anti-SARS Nucleocapsid Chimera Mab,then a FITC-conjugated second step antibody.)

Related Product Information for anti-NP-CoV antibody

Background: Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a test tool.

References

1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6. Li W. et al., 2005, Science. 309 (5742): 1864-8.