Mouse SARS-CoV-2 (2019-nCoV) Nucleocapsid Monoclonal Antibody | anti-NP-CoV antibody

SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Mouse MAb

Cat. Num. AAA8119522

• Reactivity: SARS-CoV-2
• Applications: Western Blot, ELISA, ELISA
• Purity: Protein A
• Synonyms: SARS-CoV-2 (2019-nCoV) Nucleocapsid; Monoclonal Antibody; SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody; Mouse MAb; anti-NP-CoV antibody

• Host: Mouse
• Reactivity: SARS-CoV-2
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 123
• Specificity: SARS-CoV-2 (2019-nCoV) NP Protein
• Purity/Purification: Protein A
• Form/Format: Liquid. 0.2um filtered solution in PBS

• Applicable Applications for anti-NP-CoV antibody: Western Blot (WB), ELISA (EIA), ELISA (EIA)(Cap)
• Application Notes: WB: 1:1000-1:5000; ELISA: 1:1000-1:2000; ELISA(Cap): 1:250-1:2000; Antibody's applications haven't been validated with corresponding virus positive samples.
• Immunogen: Recombinant SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein
• Conjugation: Unconjugated
• Cross-reactivity: Has minimal cross-reactivity in WB and ELISA with; SARS-CoV NP Protein ; No cross-reactivity in ELISA with; MERS-CoV NP Protein ; HCoV-229E NP Protein ; HCoV-NL63 NP Protein ; HCoV-HKU1 NP Protein ; HCoV-OC43 NP Protein
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, SARS-CoV-2 (2019-nCoV) Nucleocapsid; Met1-Ala419). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Notes: ELISA(Cap): In a sandwich ELISA, it can be used as capture antibody.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.; This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Western Blot (WB)

(Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid mouse monoclonal antibody at 1:1000 dilution. Lane A:SARS-CoV Nucleocapsid Protein (30ng) Lane B: SARS-CoV Nucleocapsid Protein (10ng) Lane C: SARS-CoV-2 (2019-nCoV) Nucleocapsid-His recombinant Protein (10ng) Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions.)

Related Product Information for anti-NP-CoV antibody

Background: Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a test tool.

References

1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6. Li W. et al., 2005, Science. 309 (5742): 1864-8.