Rabbit SARS-CoV-2 (2019-nCoV) Nucleocapsid Monoclonal Antibody | anti-NP-CoV antibody

SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Rabbit MAb

Cat. Num. AAA8119526

• Reactivity: SARS-CoV-2
• Applications: Western Blot, ELISA
• Purity: Protein A
• Synonyms: SARS-CoV-2 (2019-nCoV) Nucleocapsid; Monoclonal Antibody; SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody; Rabbit MAb; anti-NP-CoV antibody

• Host: Rabbit
• Reactivity: SARS-CoV-2
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 4
• Specificity: SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein
• Purity/Purification: Protein A
• Form/Format: Liquid. 0.2um filtered solution in PBS

• Applicable Applications for anti-NP-CoV antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; Antibody's applications have not been validated with corresponding viruses.
• Immunogen: Recombinant SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein
• Conjugation: Unconjugated
• Preparation: This product is a recombinant monoclonal antibody expressed from HEK293 cells.
• Cross-reactivity: Has cross-reactivity in ELISA and WB with; MERS-CoV Nucleocapsid Protein (His Tag) ; Has cross-reactivity in ELISA with; SARS-CoV Nucleocapsid Protein (His Tag) ; No cross-reactivity in ELISA withHCoV-229E Nucleoprotein protein ; HCoV-NL63 Nucleoprotein protein ; HCoV-HKU1(isolate N5) Nucleoprotein protein ; HCoV-OC43 Nucleoprotein ; Influenza A H1N1 (A/California/07/2009) Nucleocapsid Protein (His Tag); Influenza A H3N2 (A/Switzerland/9715293/2013) Nucleocapsid Protein (His Tag); Influenza A H2N2 Nucleocapsid Protein (His Tag); Influenza A H7N9 (A/Shanghai/2/2013) Nucleocapsid Protein (His Tag); )Influenza B (B/Florida/4/2006) Nucleocapsid Protein (His Tag)
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.; This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Western Blot (WB)

(Anti-SARS-NP rabbit monoclonal antibody at 1:1000 dilution. Lane A: SARS-CoV-2 (2019-nCoV) Nucleocapsid (40588-V08B) (30ng) Lane B: MERS-CoV Nucleocapsid (40068-V08B) (30ng) Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions.)

Related Product Information for anti-NP-CoV antibody

Background: Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a test tool.

References

1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6. Li W. et al., 2005, Science. 309 (5742): 1864-8.