Western Blot (WB)
(Figure 1: Western blot analysis using NEFH mAb against human NEFH recombinant protein. (Expected MW is 31.2 kDa))
Mouse anti-Human NEFH Monoclonal Antibody | anti-NEFH antibody
NEFH Antibody
Gene Names
NEFH; NFH; CMT2CC
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity-Chromatography
Synonyms
NEFH; Monoclonal Antibody; NEFH Antibody; 200 kDa neurofilament protein; CMT2CC; Nefh; Neurofilament heavy polypeptide 200kDa; Neurofilament heavy polypeptide; Neurofilament triplet H protein; NF H; NF-H; NFH; NFH_HUMAN; anti-NEFH antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
NEFH antibody detects endogenous levels of total NEFH
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1020
Applicable Applications for anti-NEFH antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
ELISA: 1:10000
WB: 1:500-1:2000
IHC: 1:200-1:1000
WB: 1:500-1:2000
IHC: 1:200-1:1000
Immunogen
Purified recombinant fragment of human NEFH expressed in E. Coli
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Figure 1: Western blot analysis using NEFH mAb against human NEFH recombinant protein. (Expected MW is 31.2 kDa))
Western Blot (WB)
(Figure 1: Western blot analysis using NEFH mAb against human NEFH recombinant protein. (Expected MW is 31.2 kDa))
Related Product Information for anti-NEFH antibody
Description: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein.
Function: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
Post-translational Modifications: There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.
Similarity: Belongs to the intermediate filament family.
Function: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
Post-translational Modifications: There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.
Similarity: Belongs to the intermediate filament family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 112.4 kDa
Predicted: 113 kDa
Predicted: 113 kDa
NCBI Official Full Name
neurofilament heavy polypeptide
NCBI Official Synonym Full Names
neurofilament heavy
NCBI Official Symbol
NEFH
NCBI Official Synonym Symbols
NFH; CMT2CC
NCBI Protein Information
neurofilament heavy polypeptide
UniProt Protein Name
Neurofilament heavy polypeptide
Protein Family
UniProt Gene Name
NEFH
UniProt Synonym Gene Names
KIAA0845; NFH; NF-H
NCBI Description
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008]
Uniprot Description
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
Research Articles on NEFH
Similar Products
Product Notes
The NEFH nefh (Catalog #AAA9602306) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NEFH Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NEFH can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). ELISA: 1:10000 WB: 1:500-1:2000 IHC: 1:200-1:1000. Researchers should empirically determine the suitability of the NEFH nefh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEFH, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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