Immunohistochemistry (IHC)
(Immunochemical staining of mouse CD180 in mouse thymus with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).)
Rabbit anti-Mouse RP105/CD180 Monoclonal Antibody | anti-CD180 antibody
Recombinant Anti-RP105/CD180 Antibody, Rabbit Monoclonal
Gene Names
Cd180; Ly78; RP105; F630107B15
Reactivity
Mouse
Applications
ELISA, Immunohistochemistry
Purity
Protein A
Synonyms
RP105/CD180; Monoclonal Antibody; Recombinant Anti-RP105/CD180 Antibody; Rabbit Monoclonal; CD180/RP105 Antibody; Rabbit MAb; CD180 molecule; Anti-F630107B15 Antibody; Anti-Ly78 Antibody; Anti-RP105 Antibody; anti-CD180 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
105
Specificity
Mouse CD180/RP105
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CD180 antibody
ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
ELISA: 1:5000-1:10,000
IHC-P: 1:50-1:200
IHC-P: 1:50-1:200
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Mouse CD180/RP105 Protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD180/RP105 (rM CD180/RP105).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of mouse CD180 in mouse thymus with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse CD180 in mouse thymus with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse CD180 in mouse spleen with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse CD180 in mouse spleen with rabbit monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-CD180 antibody
The cluster of differentiation (CD) system is commonly used as cell markers in immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD180, also known as RP105, is a B-cell surface molecule belonging to the family of pathogen receptors, Toll-like receptors (TLR). CD180 has an extracellular leucine-rich repeats and a short cytoplasmic tail. CD180/RP105 interacts with an extracellular molecule named MD1 and then together form the cell surface receptor complex RP105/MD1 which induces B-cell activation in humans and mice, leading to proliferation and up-regulation of a costimulatory molecule, B7.2/CD86. CD180/RP105 also has a role in LPS response because B cells lacking RP105 show hyporesponsiveness to LPS.
References
Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.Nagai Y, et al. (2002) Requirement for MD-1 in cell surface expression of RP105/CD180 and B-cell responsiveness to lipopolysaccharide. Journal of the American society of hematology. 99(5): 1699-705.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
74,302 Da
NCBI Official Full Name
CD180 antigen
NCBI Official Synonym Full Names
CD180 antigen
NCBI Official Symbol
Cd180
NCBI Official Synonym Symbols
Ly78; RP105; F630107B15
NCBI Protein Information
CD180 antigen; ly-78; lymphocyte antigen 78; radioprotective 105 kDa protein
UniProt Protein Name
CD180 antigen
Protein Family
UniProt Gene Name
Cd180
UniProt Synonym Gene Names
Ly78; Rp105; Ly-78
UniProt Entry Name
CD180_MOUSE
Similar Products
Product Notes
The CD180 cd180 (Catalog #AAA8111654) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-RP105/CD180 Antibody, Rabbit Monoclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RP105/CD180 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P). ELISA: 1:5000-1:10,000 IHC-P: 1:50-1:200. Researchers should empirically determine the suitability of the CD180 cd180 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RP105/CD180, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
