ROCK2 Polyclonal Antibody | anti-ROCK2 antibody

Anti-ROCK2 Antibody

Cat. Num. AAA177720

• Gene Names: ROCK2; ROCK-II
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: ROCK2; Polyclonal Antibody; Anti-ROCK2 Antibody; Rho-associated protein kinase 2; coiled-coil-containing protein kinase 2; KIAA0619; p164 ROCK 2; p164 ROCK-2; Rho associated coiled coil containing protein kinase 2; Rho associated protein kinase 2; Rho associated; coiled coil containing protein kinase II; Rho kinase 2; Rho-associated; ROCK 2; Rock II; Rock2; ROCK2_HUMAN; Rock2m; ROK alpha; ROKalpha; coiled-coil containing protein kinase 2; anti-ROCK2 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 1388

• Applicable Applications for anti-ROCK2 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: E Coli-derived human ROCK2 recombinant protein (Position: E652-D923). Human ROCK2 shares 94.9% and 95.2% amino acid (aa) sequence identity with mouse and rat ROCK2, respectively.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- ROCK2 Picoband antibody, MBS177720, Western blottingAll lanes: Anti ROCK2 (MBS177720) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Liver Tissue Lysate at 50ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: CEM Whole Cell Lysate at 40ugLane 5: A549 Whole Cell Lysate at 40ugLane 6: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 161KDObserved bind size: 161KD)

Immunohistochemistry (IHC)

(Anti- ROCK2 Picoband antibody, MBS177720,IHC(P)IHC(P): Mouse Cardiac Muscle Tissue )

Immunohistochemistry (IHC)

(Anti- ROCK2 Picoband antibody, MBS177720,IHC(P)IHC(P): Rat Brain Tissue )

Immunohistochemistry (IHC)

(Anti- ROCK2 Picoband antibody, MBS177720,IHC(P)IHC(P): Human Mammary Cancer Tissue )

Related Product Information for anti-ROCK2 antibody

Description: Rabbit IgG polyclonal antibody for Rho-associated protein kinase 2(ROCK2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Background: Rho-associated kinase (ROCK), including the ROCK-I and ROCK-II isoforms, is a protein kinase involved in signaling from Rho to actin cytoskeleton. Serine/threonine kinase ROCK II/Rho kinase, which is an isozyme of ROCK I, is one of the targets for the small GTPase Rho. ROCK II regulates the formation of actin stress fibers and focal adhesions, cytokinesis, smooth muscle contraction, and the activation of c-fos serum response element. Sequencing analysis has shown that human ROCK II contains 1388 amino acid residues with a calculated molecular mass of approximately 161 kDa. Fluorescence in situ hybridization analysis showed that the human ROCK II gene is located on chromosome 2p24. Thumkeo et al. concluded that ROCK-II is essential in inhibiting blood coagulation and maintaining blood flow in the endothelium-free labyrinth layer and that loss of ROCK-II leads to thrombus formation, placental dysfunction, intrauterine growth retardation, and fetal death.

References

1. Takahashi, N.; Tuiki, H.; Saya, H.; Kaibuchi, K. : Localization of the gene coding for ROCK II/Rho kinase on human chromosome 2p24. Genomics 55: 235-237, 1999. 2. Thumkeo, D.; Keel, J.; Ishizaki, T.; Hirose, M.; Nonomura, K.; Oshima, H.; Oshima, M.; Taketo, M. M.; Narumiya, S. : Targeted disruption of the mouse Rho-associated kinase 2 gene results in intrauterine growth retardation and fetal death. Molec. Cell. Biol. 23: 5043-5055, 2003.

NCBI and Uniprot Product Information

• NCBI GI #: 41872583
• NCBI GeneID: 9475
• NCBI Accession #: NP_004841.2
• NCBI GenBank Nucleotide #: NM_004850.4
• UniProt Accession #: O75116; Q53QZ0; Q53SJ7; Q9UQN5
• Molecular Weight: 160,900 Da
• NCBI Official Full Name: rho-associated protein kinase 2 isoform 1
• NCBI Official Synonym Full Names: Rho associated coiled-coil containing protein kinase 2
• NCBI Official Symbol: ROCK2
• NCBI Official Synonym Symbols: ROCK-II
• NCBI Protein Information: rho-associated protein kinase 2
• UniProt Protein Name: Rho-associated protein kinase 2
• Protein Family: Rho-associated protein kinase
• UniProt Gene Name: ROCK2
• UniProt Synonym Gene Names: KIAA0619; ROCK-II
• UniProt Entry Name: ROCK2_HUMAN

NCBI Description

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008]

Uniprot Description

ROCK2: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Homodimer. Interacts with IRS1, RHOB and RHOC. Interacts with RHOA (activated by GTP), PPP1R12A, CHORDC1, SORL1, EP300 and BRCA2. Interacts with NPM1 and this interaction enhances its activity. Interacts with RAF1. Activated by RHOA binding. Inhibited by Y-27632. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Protein type: Protein kinase, AGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; AGC group; DMPK family; ROCK subfamily

Chromosomal Location of Human Ortholog: 2p24

Cellular Component: centrosome; cytosol; nucleus; plasma membrane

Molecular Function: ATP binding; metal ion binding; protein binding; protein serine/threonine kinase activity; Rho GTPase binding; structural molecule activity

Biological Process: centrosome duplication; cortical actin cytoskeleton organization and biogenesis; cytokinesis; ephrin receptor signaling pathway; I-kappaB kinase/NF-kappaB cascade; negative regulation of angiogenesis; positive regulation of protein amino acid phosphorylation; protein amino acid phosphorylation; regulation of actin cytoskeleton organization and biogenesis; regulation of cell adhesion; regulation of circadian rhythm; regulation of focal adhesion formation; regulation of keratinocyte differentiation; regulation of stress fiber formation; Rho protein signal transduction; rhythmic process; smooth muscle contraction; vascular endothelial growth factor receptor signaling pathway