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Western Blot (WB) (Western blot analysis of DLGAP1 Antibody (N-term) in MDA-MB231 cell line lysates (35ug/lane). DLGAP1 (arrow) was detected using the purified Pab.)

Rabbit anti-Human DLGAP1 Polyclonal Antibody | anti-DLGAP1 antibody

DLGAP1, NT (Disks Large-associated Protein 1, DAP1, DAP-1, Guanylate Kinase-associated Protein, GKAP, hGKAP, MGC88156, PSD-95/SAP90-binding Protein 1, SAP90/PSD-95-associated Protein 1, SAPAP1) (MaxLight 750)

Gene Names
NPHS1; CNF; NPHN; nephrin
Reactivity
Human
Applications
Western Blot
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
DLGAP1; Polyclonal Antibody; NT (Disks Large-associated Protein 1; DAP1; DAP-1; Guanylate Kinase-associated Protein; GKAP; hGKAP; MGC88156; PSD-95/SAP90-binding Protein 1; SAP90/PSD-95-associated Protein 1; SAPAP1) (MaxLight 750); Discs; Large (Drosophila) Homolog-associated Protein 1; DAP-1-alpha; DAP-1-beta; anti-DLGAP1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human DLGAP1.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
Applicable Applications for anti-DLGAP1 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:50-1:100
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the N-terminal region of human DLGAP1 (KLH).
Conjugate
MaxLight750
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of DLGAP1 Antibody (N-term) in MDA-MB231 cell line lysates (35ug/lane). DLGAP1 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of DLGAP1 Antibody (N-term) in MDA-MB231 cell line lysates (35ug/lane). DLGAP1 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of MDA-231 cells using DLGAP1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of MDA-231 cells using DLGAP1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-DLGAP1 antibody
References
1. Ballif, B.A., et.al., Mol. Cell Proteomics 3(11), 1093-1101 (2004).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
130,546 Da
NCBI Official Full Name
nephrin
NCBI Official Synonym Full Names
nephrosis 1, congenital, Finnish type (nephrin)
NCBI Official Symbol
NPHS1
NCBI Official Synonym Symbols
CNF; NPHN; nephrin
NCBI Protein Information
nephrin; renal glomerulus-specific cell adhesion receptor
UniProt Protein Name
Disks large-associated protein 1
UniProt Gene Name
DLGAP1
UniProt Synonym Gene Names
DAP1; GKAP; DAP-1; hGKAP; SAPAP1
UniProt Entry Name
DLGP1_HUMAN

NCBI Description

This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009]

Uniprot Description

SAPAP1: Part of the postsynaptic scaffold in neuronal cells. Belongs to the SAPAP family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 18p11.31

Cellular Component: postsynaptic membrane; cell junction

Molecular Function: protein binding

Biological Process: synaptic transmission

Research Articles on DLGAP1

Similar Products

Product Notes

The DLGAP1 dlgap1 (Catalog #AAA6472535) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DLGAP1, NT (Disks Large-associated Protein 1, DAP1, DAP-1, Guanylate Kinase-associated Protein, GKAP, hGKAP, MGC88156, PSD-95/SAP90-binding Protein 1, SAP90/PSD-95-associated Protein 1, SAPAP1) (MaxLight 750) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DLGAP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:50-1:100 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the DLGAP1 dlgap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DLGAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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