Mouse anti-Human RND3 Monoclonal Antibody | anti-RND3 antibody

RND3 (Rho-related GTP-binding Protein RhoE, Protein MemB, Rho Family GTPase 3, Rho-related GTP-binding Protein Rho8, Rnd3, ARHE, RHO8, RHOE)

Cat. Num. AAA6003450

• Gene Names: RND3; ARHE; Rho8; RhoE; memB
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: RND3; Monoclonal Antibody; RND3 (Rho-related GTP-binding Protein RhoE; Protein MemB; Rho Family GTPase 3; Rho-related GTP-binding Protein Rho8; Rnd3; ARHE; RHO8; RHOE); Anti -RND3 (Rho-related GTP-binding Protein RhoE; anti-RND3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 1D2
• Specificity: Recognizes human RND3.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.

• Applicable Applications for anti-RND3 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml; Western Blot: 1:500-1:1000
• Immunogen: Recombinant corresponding to aa1-245 from human RND3 with GST tag. MW of the GST tag alone is 26kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE))

Testing Data

(Detection limit for recombinant GST tagged RND3 is 0.3ng/ml as a capture antibody.)

Related Product Information for anti-RND3 antibody

Members of the Rho family of Ras-related GTPases, such as ARHE, regulate the organization of the actin cytoskeleton in response to extracellular growth factors. Like Ras (MIM 190020), Rho family members appear to cycle between an inactive GDP-bound form and an active GTP-bound form. Three major regulators of Rho activity have been identified: RhoGDIs, which interact with the GDP-bound Rho proteins to keep them in a resting complex (see MIM 601925); GEFs, which promote GDP/GTP exchange leading to activation of Rho proteins (see MIM 601855); and GAPs, which stimulate GTP hydrolysis and return the activated Rho protein to its inactive form (see MIM 602680) (Nobes et al., 1998 [PubMed 9531558]).

Product Categories/Family for anti-RND3 antibody

Antibodies; Abs to GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 362999085
• NCBI GeneID: 390
• NCBI Accession #: NP_001241667.1
• NCBI GenBank Nucleotide #: NM_001254738.1
• UniProt Accession #: P61587; P52199; D3DP95
• Molecular Weight: 27,368 Da
• NCBI Official Full Name: rho-related GTP-binding protein RhoE
• NCBI Official Synonym Full Names: Rho family GTPase 3
• NCBI Official Symbol: RND3
• NCBI Official Synonym Symbols: ARHE; Rho8; RhoE; memB
• NCBI Protein Information: rho-related GTP-binding protein RhoE; protein MemB; small GTP binding protein Rho8; ras homolog gene family, member E; rho-related GTP-binding protein Rho8
• UniProt Protein Name: Rho-related GTP-binding protein RhoE
• Protein Family: Rho-related GTP-binding protein
• UniProt Gene Name: RND3
• UniProt Synonym Gene Names: ARHE; RHO8; RHOE
• UniProt Entry Name: RND3_HUMAN

NCBI Description

This gene encodes a protein which is a member of the small GTPase protein superfamily. The encoded protein binds only GTP but has no GTPase activity, and appears to act as a negative regulator of cytoskeletal organization leading to loss of adhesion. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Dec 2011]

Uniprot Description

RhoE: Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins. Binds ROCK1. Interacts with UBXD5. Ubiquitous. Belongs to the small GTPase superfamily. Rho family.

Protein type: Motility/polarity/chemotaxis; G protein, monomeric, Rho; G protein; G protein, monomeric

Chromosomal Location of Human Ortholog: 2q23.3

Cellular Component: Golgi membrane; focal adhesion

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: metabolic process; small GTPase mediated signal transduction; cell adhesion; actin cytoskeleton organization and biogenesis