Mouse anti-Human RHOE / RND3 Monoclonal Antibody | anti-RHOE antibody

Mouse Monoclonal [clone 1D2] (IgG2a,k) to Human RHOE / RND3

Cat. Num. AAA246218

• Gene Names: RND3; ARHE; Rho8; RhoE; memB
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: RHOE / RND3; Monoclonal Antibody; Mouse Monoclonal [clone 1D2] (IgG2a; k) to Human RHOE / RND3; Anti-RHOE / RND3 Antibody (clone 1D2) IHC-plus; RND3; ARHE; Rho family GTPase 3; RhoE; Small GTP binding protein Rho8; MemB; Rho8; Protein MemB; Human RHOE; anti-RHOE antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 1D2
• Specificity: Human RND3
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.4. Sourced in Ascites.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 244

• Applicable Applications for anti-RHOE antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (10 ug/ml), WB (1:500 - 1:1000); Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Recombinant protein.
• Target Species: Human
• Immunogen Description: RND3 (AAH12513, 1 a.a. ~ 245 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: RHOE / RND3 antibody was raised against rND3 (AAH12513, 1 a.a. ~ 245 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Aliquot and store at -20 degree C or -80 degree C. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-RND3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

ELISA (EIA)

(Detection limit for recombinant GST tagged RND3 is 0.3 ng/ml as a capture antibody.)

Product Categories/Family for anti-RHOE antibody

Family: Ras GTPase superfamily IPR001806

NCBI and Uniprot Product Information

• NCBI GI #: 4885069
• NCBI GeneID: 390
• NCBI Accession #: NP_005159.1
• NCBI GenBank Nucleotide #: NM_005168.4
• UniProt Accession #: P61587; P52199; D3DP95
• Molecular Weight: 27,368 Da
• NCBI Official Full Name: rho-related GTP-binding protein RhoE
• NCBI Official Synonym Full Names: Rho family GTPase 3
• NCBI Official Symbol: RND3
• NCBI Official Synonym Symbols: ARHE; Rho8; RhoE; memB
• NCBI Protein Information: rho-related GTP-binding protein RhoE; protein MemB; small GTP binding protein Rho8; ras homolog gene family, member E; rho-related GTP-binding protein Rho8
• UniProt Protein Name: Rho-related GTP-binding protein RhoE
• UniProt Gene Name: RND3
• UniProt Synonym Gene Names: ARHE; RHO8; RHOE
• UniProt Entry Name: RND3_HUMAN

NCBI Description

This gene encodes a protein which is a member of the small GTPase protein superfamily. The encoded protein binds only GTP but has no GTPase activity, and appears to act as a negative regulator of cytoskeletal organization leading to loss of adhesion. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Dec 2011]

Uniprot Description

RhoE: Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins. Binds ROCK1. Interacts with UBXD5. Ubiquitous. Belongs to the small GTPase superfamily. Rho family.

Protein type: G protein; Motility/polarity/chemotaxis; G protein, monomeric; G protein, monomeric, Rho

Chromosomal Location of Human Ortholog: 2q23.3

Cellular Component: Golgi membrane; focal adhesion

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: metabolic process; small GTPase mediated signal transduction; cell adhesion; actin cytoskeleton organization and biogenesis