Rabbit ARHE Polyclonal Antibody | anti-RND3 antibody

ARHE Antibody (Center)

Cat. Num. AAA9209800

• Gene Names: RND3; ARHE; Rho8; RhoE; memB
• Reactivity: Human, mouse (Predicted Reactivity: Pig, Rat)
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: ARHE; Polyclonal Antibody; ARHE Antibody (Center); Rho-related GTP-binding protein RhoE; Protein MemB; Rho family GTPase 3; Rho-related GTP-binding protein Rho8; Rnd3; RND3; RHO8; RHOE; anti-RND3 antibody

• Host: Rabbit
• Reactivity: Human, mouse (Predicted Reactivity: Pig, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This ARHE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-165 amino acids from the Central region of human ARHE.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 133-165
• Sequence Length: 244

• Applicable Applications for anti-RND3 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of ARHE Antibody (Center) in mouse brain tissue lysates (35ug/lane). ARHE (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ARHE antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-RND3 antibody

Members of the Rho family of Ras-related GTPases, such as ARHE, regulate the organization of the actin cytoskeleton in response to extracellular growth factors. Like Ras (MIM 190020), Rho family members appear to cycle between an inactive GDP-bound form and an active GTP-bound form. Three major regulators of Rho activity have been identified: RhoGDIs, which interact with the GDP-bound Rho proteins to keep them in a resting complex (see MIM 601925); GEFs, which promote GDP/GTP exchange leading to activation of Rho proteins (see MIM 601855); and GAPs, which stimulate GTP hydrolysis and return the activated Rho protein to its inactive form (see MIM 602680) (Nobes et al., 1998 [PubMed 9531558]).

Product Categories/Family for anti-RND3 antibody

Cancer; Signal Transduction

References

Pinner,S., Nat. Cell Biol. 10 (2), 127-137 (2008)
Poch,E., Exp. Cell Res. 313 (4), 719-731 (2007)
Ongusaha,P.P., Curr. Biol. 16 (24), 2466-2472 (2006)

NCBI and Uniprot Product Information

• NCBI GI #: 362999085
• NCBI GeneID: 390
• NCBI Accession #: NP_001241667.1; NP_005159.1
• NCBI GenBank Nucleotide #: NM_001254738.1
• UniProt Accession #: P61587; P52199; D3DP95
• Molecular Weight: 27368
• NCBI Official Full Name: rho-related GTP-binding protein RhoE
• NCBI Official Synonym Full Names: Rho family GTPase 3
• NCBI Official Symbol: RND3
• NCBI Official Synonym Symbols: ARHE; Rho8; RhoE; memB
• NCBI Protein Information: rho-related GTP-binding protein RhoE
• UniProt Protein Name: Rho-related GTP-binding protein RhoE
• Protein Family: Rho-related GTP-binding protein
• UniProt Gene Name: RND3
• UniProt Synonym Gene Names: ARHE; RHO8; RHOE
• UniProt Entry Name: RND3_HUMAN

NCBI Description

This gene encodes a protein which is a member of the small GTPase protein superfamily. The encoded protein binds only GTP but has no GTPase activity, and appears to act as a negative regulator of cytoskeletal organization leading to loss of adhesion. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Dec 2011]

Uniprot Description

RhoE: Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins. Binds ROCK1. Interacts with UBXD5. Ubiquitous. Belongs to the small GTPase superfamily. Rho family.

Protein type: G protein, monomeric, Rho; G protein; Motility/polarity/chemotaxis; G protein, monomeric

Chromosomal Location of Human Ortholog: 2q23.3

Cellular Component: Golgi membrane; focal adhesion

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: metabolic process; small GTPase mediated signal transduction; cell adhesion; actin cytoskeleton organization and biogenesis