Testing Data
(Detection of Rit2: Human cerebellum and hypothalamus tissue lysates were resolved by SDS-PAGE. Following electrophoresis, proteins were transferred to an Immobilon-P membrane and immunoblotted with 2ug/ml mouse anti-Rit2)
Mouse anti-Human RIT2 Monoclonal Antibody
RIT2 (Ras-like Protein Expressed in Neurons, Ras-like Without CAAX Protein 2, RIN, ROC2)
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
RIT2; Monoclonal Antibody; RIT2 (Ras-like Protein Expressed in Neurons; Ras-like Without CAAX Protein 2; RIN; ROC2); Anti -RIT2 (Ras-like Protein Expressed in Neurons; anti-RIT2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
11C49
Specificity
Recognizes human Rit2.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.
Applicable Applications for anti-RIT2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Immunohistochemistry and Western Blot.
Dilution: Immunohistochemistry: 25ug/ml
Western Blot: 2ug/ml
Dilution: Immunohistochemistry: 25ug/ml
Western Blot: 2ug/ml
Immunogen
Purified, E. coli-derived full-length recombinant human Rit2 (aa1-217; Accession # Q99578).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(Detection of Rit2: Human cerebellum and hypothalamus tissue lysates were resolved by SDS-PAGE. Following electrophoresis, proteins were transferred to an Immobilon-P membrane and immunoblotted with 2ug/ml mouse anti-Rit2)
Testing Data
(Detection of Rit2: Human cerebellum and hypothalamus tissue lysates were resolved by SDS-PAGE. Following electrophoresis, proteins were transferred to an Immobilon-P membrane and immunoblotted with 2ug/ml mouse anti-Rit2)
Related Product Information for anti-RIT2 antibody
GTP-binding protein Rit2 (Rit2; also Rin, Ras-like protein expressed in neurons, Ras-like without CAAX) is a 25kD member of the small GTPase superfamily and Ras family. Human Rit2 is 217aa in length. Like all small G-proteins, Rit2 contains five highly conserved domains (G1-G5) and acts as a molecular switch by alternating an active GTP-bound form and an inactive GDP-bound form. There are two isoforms for Rit2. Isoform 1 is the long form. Isoform 2 has an 11aa substitution for residues 143-153 found in isoform 1 and a deletion of aa154-217. Rit2 is expressed exclusively in neurons. In addition, Rit2 binds calmodulin through a C-terminal binding motif.
Product Categories/Family for anti-RIT2 antibody
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Product Notes
The RIT2 (Catalog #AAA631823) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RIT2 (Ras-like Protein Expressed in Neurons, Ras-like Without CAAX Protein 2, RIN, ROC2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RIT2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Immunohistochemistry and Western Blot. Dilution: Immunohistochemistry: 25ug/ml Western Blot: 2ug/ml. Researchers should empirically determine the suitability of the RIT2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RIT2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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