Rabbit anti-Human RIT2 Polyclonal Antibody | anti-RIT2 antibody

RIT2, NT (RIT2, RIN, ROC2, GTP-binding protein Rit2, Ras-like protein expressed in neurons, Ras-like without CAAX protein 2) (FITC)

Cat. Num. AAA6342217

• Gene Names: RIT2; RIN; RIBA; ROC2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RIT2; Polyclonal Antibody; NT (RIT2; RIN; ROC2; GTP-binding protein Rit2; Ras-like protein expressed in neurons; Ras-like without CAAX protein 2) (FITC); Ras-like without CAAX protein 2; anti-RIT2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-RIT2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RIT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-69 amino acids from the N-terminal region of human RIT2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RIT2 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the RIT2 antibody detected the RIT2 protein (arrow).)

Related Product Information for anti-RIT2 antibody

RIN belongs to the RAS (HRAS; MIM 190020) superfamily of small GTPases (Shao et al., 1999 [PubMed 10545207]).[supplied by OMIM].

Product Categories/Family for anti-RIT2 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Glessner, J.T., et al. Proc. Natl. Acad. Sci. U.S.A. 107(23):10584-10589(2010). Need, A.C., et al. Hum. Mol. Genet. 18(23):4650-4661(2009). Sonuga-Barke, E.J., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 147B (8), 1359-1368 (2008): Wu, C., et al. Proteomics 7(11):1775-1785(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4506533
• NCBI GeneID: 6014
• NCBI Accession #: NP_002921.1
• NCBI GenBank Nucleotide #: NM_002930.3
• UniProt Accession #: Q99578; O15295; Q8TD69; Q8WVF6; Q92964; B2R9L1
• Molecular Weight: 17,308 Da
• NCBI Official Full Name: GTP-binding protein Rit2 isoform 1
• NCBI Official Synonym Full Names: Ras-like without CAAX 2
• NCBI Official Symbol: RIT2
• NCBI Official Synonym Symbols: RIN; RIBA; ROC2
• NCBI Protein Information: GTP-binding protein Rit2; GTP-binding protein Roc2; Ric-like, expressed in neurons; ras-like without CAAX protein 2; ras-like protein expressed in neurons
• UniProt Protein Name: GTP-binding protein Rit2
• Protein Family: GTP-binding protein
• UniProt Gene Name: RIT2
• UniProt Synonym Gene Names: RIN; ROC2
• UniProt Entry Name: RIT2_HUMAN

NCBI Description

RIN belongs to the RAS (HRAS; MIM 190020) superfamily of small GTPases (Shao et al., 1999 [PubMed 10545207]).[supplied by OMIM, Mar 2008]

Uniprot Description

RIT2: Binds and exchanges GTP and GDP. Belongs to the small GTPase superfamily. Ras family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein; G protein, monomeric, Ras; G protein, monomeric

Chromosomal Location of Human Ortholog: 18q12.3

Cellular Component: plasma membrane; nucleus

Molecular Function: GTPase activity; calmodulin binding; GTP binding

Biological Process: synaptic transmission; nerve growth factor receptor signaling pathway; metabolic process; small GTPase mediated signal transduction; signal transduction