Rabbit anti-Human BIM Polyclonal Antibody | anti-BIM antibody

BIM (BOD) (PE)

Cat. Num. AAA6465295

• Gene Names: BCL2L11; BAM; BIM; BOD
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography from antisera.
• Synonyms: BIM; Polyclonal Antibody; BIM (BOD) (PE); anti-BIM antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BIM.
• Purity/Purification: Purified by affinity chromatography from antisera.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-BIM antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: Paraffin sectiosn. Requires antigen retrival using heat treatment prior to staining of paraffin sections. Sodium citrate pH 6 is recommended for antigen retrival.; WB: detects a band of ~22kD in K562 and A549 whole cell lysates.; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to aa22-40 of human BIM. Species sequence homology: mouse, rat
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of whole cell lysates from K562 (K) and A549 (A) cells probed with B1090-04.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of human skin with B1090-04.)

Related Product Information for anti-BIM antibody

Bim is a pro-apoptotic protein within the Bcl-2 family, which functions within the intrinsic apotosis pathway. Bim is known to interact with pro-survival proteins including Bcl-2, Bcl-XL and Bcl-w. Bim is expressed in a variety of cell types, where it is maintained in an inactive conformation by phosphorylation or through binding to the microtubule-associated dynein motor complex. Upon certain apoptotic stimuli, Bim is released from microtubules and mediates caspase-dependent apoptosis.

Product Categories/Family for anti-BIM antibody

Antibodies; Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 323362959
• NCBI GeneID: 10018
• NCBI Accession #: NP_001191035.1
• NCBI GenBank Nucleotide #: NM_001204106.1
• UniProt Accession #: O43521; O43522; Q0MSE7; Q0MSE8; Q0MSE9; Q53R28; Q6JTU6; Q6T851; Q6TE14; Q6TE15; Q6TE16; A8K2W2
• Molecular Weight: 22,171 Da
• NCBI Official Full Name: bcl-2-like protein 11 isoform 11
• NCBI Official Synonym Full Names: BCL2-like 11 (apoptosis facilitator)
• NCBI Official Symbol: BCL2L11
• NCBI Official Synonym Symbols: BAM; BIM; BOD
• NCBI Protein Information: bcl-2-like protein 11; bcl-2 interacting protein Bim; bcl-2-related ovarian death agonist; bcl-2 interacting mediator of cell death
• UniProt Protein Name: Bcl-2-like protein 11
• UniProt Gene Name: BCL2L11
• UniProt Synonym Gene Names: BIM; Bcl2-L-11
• UniProt Entry Name: B2L11_HUMAN

NCBI Description

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jun 2013]

Uniprot Description

BIM: a pro-apoptotic member of the BCL-2 protein family. Interacts with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and act as an apoptotic activator. Its expression can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role in neuronal and lymphocyte apoptosis. Transgenic studies in the mouse suggested that this protein functions as an essential initiator of the apoptosis in thymocyte-negative selection. Nineteen alternatively spliced transcript variants of this gene have been reported.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 2q13

Cellular Component: microtubule; mitochondrial outer membrane; extrinsic to membrane; endomembrane system; cytosol

Molecular Function: protein binding; microtubule binding

Biological Process: nerve growth factor receptor signaling pathway; positive regulation of apoptosis; apoptosis; cell-matrix adhesion; pigmentation during development; myeloid cell homeostasis; ear development; positive regulation of caspase activity; B cell apoptosis; cellular process regulating host cell cycle in response to virus; positive regulation of apoptosis by virus; mammary gland development; B cell homeostasis; positive regulation of neuron apoptosis; T cell homeostasis; kidney development; post-embryonic organ morphogenesis; caspase activation; spleen development; thymus development; in utero embryonic development; positive regulation of protein homooligomerization; male gonad development; positive regulation of cell cycle; regulation of organ growth; lumen formation; odontogenesis of dentine-containing teeth; DNA damage response, signal transduction resulting in induction of apoptosis; regulation of pigmentation during development; spermatogenesis; brain development