Mouse Ribonuclease P Protein Subunit p20 Monoclonal Antibody | anti-POP7 antibody

Ribonuclease P Protein Subunit p20 (RNaseP Protein p20, RPP20, 0610037N12Rik, hPOP7, Processing of Precursor 7 Ribonuclease P/MRP Subunit (S. cerevisiae), POP7, Ribonucleases P/MRP Protein Subunit POP7 Homolog, RPP2)

Cat. Num. AAA649830

• Gene Names: POP7; RPP2; RPP20; 0610037N12Rik
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Ribonuclease P Protein Subunit p20; Monoclonal Antibody; Ribonuclease P Protein Subunit p20 (RNaseP Protein p20; RPP20; 0610037N12Rik; hPOP7; Processing of Precursor 7 Ribonuclease P/MRP Subunit (S. cerevisiae); POP7; Ribonucleases P/MRP Protein Subunit POP7 Homolog; RPP2); Anti -Ribonuclease P Protein Subunit p20 (RNaseP Protein p20; anti-POP7 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 5BH5
• Specificity: Recognizes human Ribonuclease P Protein Subunit p20.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from 10mM ammonium bicarbonate buffer, 2mg BSA. Reconstitute with sterile dH2O.

• Applicable Applications for anti-POP7 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: Under less stringent washing conditions, a weaker band of ~60kD is also detected.
• Immunogen: Recombinant GST-hRPP20 (Accession #AAC24113, expression vector pGEX-2T), expressed in E.coli.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-POP7 antibody

The nuclei of eukaryotic cells contain several classes of small RNA-protein complexes. SnRNPs (small nuclear ribonucleoproteins) are involved in splicing of pre-mRNAs (the excision of introns) in the nucleoplasm. RPP20 is part of such a complex. SnoRNPs (small nucleolar ribonucleoproteins) are involved in the maturation of pre-ribosomal RNA in the nucleoli. Pre-rRNA processing and modification require both the snoRNA and the protein constituents (such as Rpp20) of these complexes. Generally, snoRNPs contain a number of common proteins, which are shared with other snoRNPs of the same family, next to several particle- specific proteins. On average, snoRNPs contain about 6-10 protein subunits.

Product Categories/Family for anti-POP7 antibody

Antibodies; Abs to Enzymes; Ribonuclease

NCBI and Uniprot Product Information

• NCBI GI #: 153791431
• NCBI GeneID: 10248
• NCBI Accession #: NP_005828.2
• NCBI GenBank Nucleotide #: NM_005837.2
• UniProt Accession #: O75817; Q9BV74; A4D2E0
• Molecular Weight: 15,651 Da
• NCBI Official Full Name: ribonuclease P protein subunit p20
• NCBI Official Synonym Full Names: processing of precursor 7, ribonuclease P/MRP subunit (S. cerevisiae)
• NCBI Official Symbol: POP7
• NCBI Official Synonym Symbols: RPP2; RPP20; 0610037N12Rik
• NCBI Protein Information: ribonuclease P protein subunit p20; hPOP7; RNaseP protein p20; ribonucleases P/MRP protein subunit POP7 homolog; processing of precursor 7, ribonuclease P subunit; POP7 (processing of precursor, S. cerevisiae) homolog
• UniProt Protein Name: Ribonuclease P protein subunit p20
• Protein Family: Ribonuclease P protein
• UniProt Gene Name: POP7
• UniProt Synonym Gene Names: RPP20; RNaseP protein p20; hPOP7
• UniProt Entry Name: POP7_HUMAN

Uniprot Description

Function: Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.

Catalytic activity: Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.

Subunit structure: Component of nuclear RNase P and RNase MRP ribonucleoproteins. RNase P consists of a RNA moiety and at least 8 protein subunits; POP1, RPP14, RPP20/POP7, RPP25, RPP29/POP4, RPP30, RPP38 and RPP40. RNase MRP consists of a RNA moiety and at least 9 protein subunits; POP1, RPP14, RPP20/POP7, RPP25, RPP29/POP4, RPP30, RPP38, RPP40, POP5 and RPP21. Interacts with RPP25. RPP20/POP7 is probably a dimer. Ref.5

Subcellular location: Nucleus › nucleolus

Potential.

Sequence similarities: Belongs to the histone-like Alba family.