Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dull epidermal staining.)
Mouse anti-Vertebrates Rhodopsin Monoclonal Antibody | anti-RHO antibody
Rhodopsin Antibody: Biotin
Reactivity
Vertebrates
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot, ELISA, Immunofluorescence
Synonyms
Rhodopsin; Monoclonal Antibody; Rhodopsin Antibody: Biotin; OPN2; opsd; opsin 2; opsin 2 rod pigment; opsin2; RHO; RP4; MGC138309; Retinitis Pigmentosa 4; anti-RHO antibody
Host
Mouse
Reactivity
Vertebrates
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1D4
Form/Format
Protein G Purified
Concentration
1mg/mL (varies by lot)
Sequence Length
348
Applicable Applications for anti-RHO antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB), ELISA (EIA), Immunofluorescence (IF)
Application Notes
1:1000 (WB)
Conjugate
Biotin
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Preparation and Storage
-20 degree C
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dull epidermal staining.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dull epidermal staining.)
Western Blot (WB)
(Western Blot analysis of Human Cell lysates showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Western Blot (WB)
(Western Blot analysis of Human Cell lysates showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Related Product Information for anti-RHO antibody
Background Info: Detects ~40kDa band corresponding to the molecular weight of Rhodopsin. Recognizes Rhodopsin (native and recombinant forms). No known reactivity to other proteins. Binds specifically to the C-terminal epitope -T-E-T-S-Q-V-A-P-A-(COOH).
Scientific Background: Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).
Scientific Background: Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).
Product Categories/Family for anti-RHO antibody
References
1. Molday R.S., Hicks D., and Molday L. (1987) Invest Ophthalmol Vis Sci. 28: 50-61. 2. Ridge K.D., Lee S.S.J., and Abdulaev N.G. (1996) J of Biol Chem. 271: 7860-7867. 3. Matsuyama T., Yamashita T., Imai H. and Shichida Y. (2009) J Biol Chem. Manuscript M109.063875. 4. Feurstein S.E., et al. (2009) Biochemistry. 48(45): 10733-10742. 5. Iannaccone A., et al. (2006) Vision Res. 46(27): 4556-4567.
1. Cheng, C.L., Djajadi, H. and Molday, R.S. (2012). Cell-Specific Markers for the Identification of Retinal Cells by Immunofluorescence Microscopy. Methods in Molecular Biology. 935, 185-199. doi: 10.1007/978-1-62703-080-9_12
1. Cheng, C.L., Djajadi, H. and Molday, R.S. (2012). Cell-Specific Markers for the Identification of Retinal Cells by Immunofluorescence Microscopy. Methods in Molecular Biology. 935, 185-199. doi: 10.1007/978-1-62703-080-9_12
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
39,008 Da
NCBI Official Full Name
rhodopsin
NCBI Official Synonym Full Names
rhodopsin<
NCBI Official Symbol
RHO
NCBI Protein Information
rhodopsin; rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant)
UniProt Protein Name
Rhodopsin
Protein Family
UniProt Gene Name
RHO
UniProt Entry Name
OPSD_BOVIN
Uniprot Description
Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal ().
Research Articles on RHO
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Product Notes
The RHO rho (Catalog #AAA802096) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Rhodopsin Antibody: Biotin reacts with Vertebrates and may cross-react with other species as described in the data sheet. AAA Biotech's Rhodopsin can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB), ELISA (EIA), Immunofluorescence (IF). 1:1000 (WB). Researchers should empirically determine the suitability of the RHO rho for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rhodopsin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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