Mouse anti-Vertebrate Rhodopsin Monoclonal Antibody | anti-RHO antibody

Rhodopsin Antibody

Cat. Num. AAA8001930

• Reactivity: Vertebrate
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, ELISA
• Purity: Protein G Purified
• Synonyms: Rhodopsin; Monoclonal Antibody; Rhodopsin Antibody; Mouse Anti-Bovine Rhodopsin Monoclonal IgG1; Clone 1D4; OPN2 Antibody; opsd Antibody; opsin 2 Antibody; opsin 2 rod pigment Antibody; opsin2 Antibody; RHO Antibody; RP4 Antibody; MGC138309 Antibody; Retinitis Pigmentosa 4 Antibody; anti-RHO antibody

• Host: Mouse
• Reactivity: Vertebrate
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1D4
• Specificity: Detects ~40kDa. Binds specifically to the N-terminus of Rhodopsin. Does not detect Rhodopsin in invertebrates.
• Purity/Purification: Protein G Purified
• Form/Format: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 348

• Applicable Applications for anti-RHO antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA (EIA)
• Application Notes: WB: 1:1000; IHC: 1:100
• Immunogen: Bovine Rhodopsin
• Immunogen Species: Bovine
• Conjugate: Unconjugated
• Cellular Localization: Membrane
• Certificate of Analysis: 1 ug/ml of SMC-177 was sufficient for detection of rhodopsin in 10 ug of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Preparation and Storage: Store at-20 degree C.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4 . Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dull epidermal staining.)

Western Blot (WB)

(Western Blot analysis of Human Cell lysates showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4 . Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

Related Product Information for anti-RHO antibody

Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).

Product Categories/Family for anti-RHO antibody

Neuroscience; Neurotransmitter Receptors; Rhodopsins; Cell Signaling

References

1. Molday R.S., Hicks D., and Molday L. (1987) Invest Ophthalmol Vis Sci. 28: 50-61. 2. Ridge K.D., Lee S.S.J., and Abdulaev N.G. (1996) J of Biol Chem. 271: 7860-7867. 3. Matsuyama T., Yamashita T., Imai H. and Shichida Y. (2009) J Biol Chem. Manuscript M109.063875. 4. Feurstein S.E., et al. (2009) Biochemistry. 48(45): 10733-10742. 5. Iannaccone A., et al. (2006) Vision Res. 46(27): 4556-4567.

NCBI and Uniprot Product Information

• NCBI GI #: 62460472
• NCBI GeneID: 509933
• NCBI Accession #: NP_001014890.1
• NCBI GenBank Nucleotide #: NM_001014890.2
• UniProt Accession #: P02699
• NCBI Official Full Name: rhodopsin
• NCBI Official Synonym Full Names: rhodopsin
• NCBI Official Symbol: RHO
• NCBI Protein Information: rhodopsin
• UniProt Protein Name: Rhodopsin
• Protein Family: Rhodopsin
• UniProt Gene Name: RHO
• UniProt Entry Name: OPSD_BOVIN

Uniprot Description

Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal ().