Mouse Rhodopsin Monoclonal Antibody | anti-RHO antibody

Rhodopsin Antibody

Cat. Num. AAA804285

• Reactivity: All mammalian species (Human, Mice, Rat, Bovine, Pig etc.), most Fish, Birds, Amphibians (Frogs, Xenopus), Anchovies. Not Drosophila or other invertebrates.
• Applications: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot, ELISA
• Synonyms: Rhodopsin; Monoclonal Antibody; Rhodopsin Antibody; OPN2; opsd; opsin 2; opsin 2 rod pigment; opsin2; RHO; RP4; MGC138309; Retinitis Pigmentosa 4; anti-RHO antibody

• Host: Mouse
• Reactivity: All mammalian species (Human, Mice, Rat, Bovine, Pig etc.), most Fish, Birds, Amphibians (Frogs, Xenopus), Anchovies. Not Drosophila or other invertebrates.
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4D2
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 348

• Applicable Applications for anti-RHO antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB), ELISA (EIA)
• Application Notes: 1:1000 (WB), 1-2ug per 100-500ug of protein (IP).
• Certificate of Analysis: 1 ug/mL was sufficient for detection of rhodopsin in 10ug of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2. Tissue: retina. Species: Mouse. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain. Localization: Staining of photoreceptor outer segment (OS). Other layers of the retina: IS - inner segment; ONL - outer nuclear layer; OPL - outer plexiform layer; INL - inner nuclear layer; IPL - inner plexiform layer; GCL - ganglion cell layer.)

Related Product Information for anti-RHO antibody

Background Info: Detects ~40kDa band corresponding to the molecular weight of Rhodopsin. Binds specifically to the N-terminus of Rhodopsin.

Scientific Background: Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).

Product Categories/Family for anti-RHO antibody

GTPase

References

1. Molday R.S., Hicks D., and Molday L. (1987) Invest Ophthalmol Vis Sci. 28: 50-61. 2. Ridge K.D., Lee S.S.J., and Abdulaev N.G. (1996) J of Biol Chem. 271: 7860-7867. 3. Matsuyama T., Yamashita T., Imai H. and Shichida Y. (2009) J Biol Chem. Manuscript M109.063875. 4. Feurstein S.E., et al. (2009) Biochemistry. 48(45): 10733-10742. 5. Iannaccone A., et al. (2006) Vision Res. 46(27): 4556-4567.
1. Cheng, C.L., Djajadi, H. and Molday, R.S. (2012). Cell-Specific Markers for the Identification of Retinal Cells by Immunofluorescence Microscopy. Methods in Molecular Biology. 935, 185-199. doi: 10.1007/978-1-62703-080-9_12

NCBI and Uniprot Product Information

• NCBI GI #: 62460472
• NCBI GeneID: 509933
• NCBI Accession #: NP_001014890.1
• NCBI GenBank Nucleotide #: NM_001014890.1
• UniProt Accession #: P02699
• Molecular Weight: 39,008 Da
• NCBI Official Full Name: rhodopsin
• NCBI Official Synonym Full Names: rhodopsin<
• NCBI Official Symbol: RHO
• NCBI Protein Information: rhodopsin; rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant)
• UniProt Protein Name: Rhodopsin
• Protein Family: Rhodopsin
• UniProt Gene Name: RHO
• UniProt Entry Name: OPSD_BOVIN

Uniprot Description

Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal ().