Rabbit anti-Human RHOB Polyclonal Antibody | anti-RHOB antibody

RHOB Antibody

Cat. Num. AAA9408400

• Gene Names: RHOB; ARH6; ARHB; RHOH6; MST081; MSTP081
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: RHOB; Polyclonal Antibody; RHOB Antibody; H6; Rho-related GTP-binding protein RhoB; ARH6; ARHB; anti-RHOB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total RHOB protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 196

• Applicable Applications for anti-RHOB antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500~1:3000
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from internal of human RHOB.
• Target Name: RHOB
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells and HeLa cells, using RHOB antibody.)

Related Product Information for anti-RHOB antibody

Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Required for genotoxic stress-induced cell death in breast cancer cells.Chardin P., Nucleic Acids Res. 16:2717-2717(1988).Puhl H.L. III, Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.Halleck A., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.

Product Categories/Family for anti-RHOB antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4757764
• NCBI GeneID: 388
• NCBI Accession #: NP_004031.1
• NCBI GenBank Nucleotide #: NM_004040.3
• UniProt Accession #: P62745; P01121; Q5U0H6; Q7RTN5; Q7RTR9; Q9CUV7; B2R692
• Molecular Weight: 22,123 Da
• NCBI Official Full Name: rho-related GTP-binding protein RhoB
• NCBI Official Synonym Full Names: ras homolog family member B
• NCBI Official Symbol: RHOB
• NCBI Official Synonym Symbols: ARH6; ARHB; RHOH6; MST081; MSTP081
• NCBI Protein Information: rho-related GTP-binding protein RhoB
• UniProt Protein Name: Rho-related GTP-binding protein RhoB
• Protein Family: Rho-related GTP-binding protein
• UniProt Gene Name: RHOB
• UniProt Synonym Gene Names: ARH6; ARHB; h6
• UniProt Entry Name: RHOB_HUMAN

Uniprot Description

RHOB: a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Controls the reorganization of actins into podosomes. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders.

Protein type: G protein, monomeric, Rho; Motility/polarity/chemotaxis; G protein, monomeric; G protein; Tumor suppressor

Chromosomal Location of Human Ortholog: 2p24

Cellular Component: focal adhesion; late endosome membrane; early endosome; plasma membrane; endosome membrane; cytosol; nucleus; cleavage furrow

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding

Biological Process: platelet activation; axon guidance; transformed cell apoptosis; apoptosis; metabolic process; positive regulation of apoptosis; cytokinesis; Rho protein signal transduction; negative regulation of cell cycle; intracellular protein transport; regulation of small GTPase mediated signal transduction; positive regulation of angiogenesis; small GTPase mediated signal transduction; endosome to lysosome transport; angiogenesis; cell adhesion; blood coagulation