Immunofluorescence (IF)
(Immunofluorescence staining of fixed K562 cells with anti-Rhesus D antigen antibody D7C2 (MBS488641) Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of D7C2 (MBS488641) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488641, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Human anti-Human Rhesus D antigen Monoclonal Antibody
Anti-Rhesus D antigen [D7C2]
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Purified antibody. Affinity Purified using a recombinant lectin column
Synonyms
Rhesus D antigen; Monoclonal Antibody; Anti-Rhesus D antigen [D7C2]; CD240D; RHXIII; Rh polypeptide 2; RhPII; anti-Rhesus D antigen antibody
Host
Human
Reactivity
Human
Clonality
Monoclonal
Isotype
Human IgM, lambda
Clone Number
D7C2
Purity/Purification
Purified antibody. Affinity Purified using a recombinant lectin column
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Rhesus D antigen antibody
Flow Cytometry (FC/FACS), Antibody-Dependent Cellular Cytotoxicity (ADCC)
Application Notes
FC confirmed the binding of D7C2 to Rh D+ red cells in artifical mixtures (Edelman et al, 1997). The ADCC of D7C2 showed a percentage of 93% lysis (Edelman et al, 1997). D7C2 has been expressed recombinantly using a baculovirus-insect cell expression system (Edelman et al, 1997).
PMID
9203960
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Immunofluorescence (IF)
(Immunofluorescence staining of fixed K562 cells with anti-Rhesus D antigen antibody D7C2 (MBS488641) Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of D7C2 (MBS488641) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488641, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed K562 cells with anti-Rhesus D antigen antibody D7C2 (MBS488641) Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of D7C2 (MBS488641) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488641, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-Rhesus D antigen antibody
Recombinant monoclonal antibody to Rhesus D antigen. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma D7C2.
A Rh- volunteer blood donor (r/r) was injected with 5ml of Rh+ erythrocytes (R1/r) and the peripheral lymphocytes were isolated and transformed with 1 ml of culture supernatant of the EBV marmoset B96-8 cell line.
A Rh- volunteer blood donor (r/r) was injected with 5ml of Rh+ erythrocytes (R1/r) and the peripheral lymphocytes were isolated and transformed with 1 ml of culture supernatant of the EBV marmoset B96-8 cell line.
Product Categories/Family for anti-Rhesus D antigen antibody
References
Edelman et al, 1997. Obtaining a functional recombinant anti-rhesus (D) antibody using the baculovirus-insect cell expression system. Immunology. 1997 May; 91(1): 13-19.
Note on publication: Describes the original production of the antibody.
Note on publication: Describes the original production of the antibody.
NCBI and Uniprot Product Information
UniProt Accession #
Similar Products
Product Notes
The Rhesus D antigen (Catalog #AAA488640) is an Antibody produced from Human and is intended for research purposes only. The product is available for immediate purchase. The Anti-Rhesus D antigen [D7C2] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Rhesus D antigen can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Antibody-Dependent Cellular Cytotoxicity (ADCC). FC confirmed the binding of D7C2 to Rh D+ red cells in artifical mixtures (Edelman et al, 1997). The ADCC of D7C2 showed a percentage of 93% lysis (Edelman et al, 1997). D7C2 has been expressed recombinantly using a baculovirus-insect cell expression system (Edelman et al, 1997). Researchers should empirically determine the suitability of the Rhesus D antigen for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rhesus D antigen, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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