Western Blot (WB)
(Western blot analysis of HEK293 cells transfected with RFP-tagged protein vector. 1:1000 (1ug/mL) Ab dilution. Non·-transfected (1). transfected with TurboRFP (2) and transfected with dsRed (3))
Mouse RFP Tag Monoclonal Antibody | anti-RFP antibody
RFP Tag Mouse Monoclonal Antibody (RF5R)
Applications
ELISA, Immunoprecipitation, Immunohistochemistry, Western Blot
Synonyms
RFP Tag; Monoclonal Antibody; RFP Tag Mouse Monoclonal Antibody (RF5R); anti-RFP antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
RF5R
Specificity
The antibody recognizes native and denatured forms of RFP and its variants: TagRFR TurboRFR dsRed, mCherry, mOrange, tdTomato, etc
Form/Format
Lyophilized protein G purified in PBS pH7.4
Applicable Applications for anti-RFP antibody
ELISA (EIA), Dot, Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
If reconstituted with deionized water in 100 uL: WB: 1:1,000-3,000; IHC: 1:500-2,000.
Optimal dilution has to be determined by the user.
Optimal dilution has to be determined by the user.
Immunogen
The antibody was raised against RFP from the Discosoma sea anemone N-terminal peptide-KLH conjugated.
Target
RFP Tag
Preparation and Storage
Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
Western Blot (WB)
(Western blot analysis of HEK293 cells transfected with RFP-tagged protein vector. 1:1000 (1ug/mL) Ab dilution. Non·-transfected (1). transfected with TurboRFP (2) and transfected with dsRed (3))
Western Blot (WB)
(Western blot analysis of HEK293 cells transfected with RFP-tagged protein vector. 1:1000 (1ug/mL) Ab dilution. Non·-transfected (1). transfected with TurboRFP (2) and transfected with dsRed (3))
Related Product Information for anti-RFP antibody
Target background: Red Fluorescent Protein (RFP) is a protein derived from the Discosoma sea anemone. Fluorescent proteins are powerful tools to study protein localization and dynamics in living cells. The anti-RFP antibody can be used to detect native RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange and tdTomato.
Similar Products
Product Notes
The RFP (Catalog #AAA588123) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's RFP Tag can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Dot, Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB). If reconstituted with deionized water in 100 uL: WB: 1:1,000-3,000; IHC: 1:500-2,000. Optimal dilution has to be determined by the user. Researchers should empirically determine the suitability of the RFP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RFP Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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