Reactivity Chart
Goat tdTomato Polyclonal Antibody | anti-tdTomato antibody
Anti-tdTomato, DyLight633
Reactivity
Transfected cells, tdTomato, mCherry, red fluorescent protein (DsRed) from so-called disc corals of Discosoma species.
Applications
Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Epitope affinity purified
Synonyms
tdTomato; Polyclonal Antibody; Anti-tdTomato; DyLight633; Cherry fluorescent protein; DsRed; mCherry; red fluorescent protein; RFP antibody; anti-tdTomato antibody
Host
Goat
Reactivity
Transfected cells, tdTomato, mCherry, red fluorescent protein (DsRed) from so-called disc corals of Discosoma species.
Clonality
Polyclonal
Isotype
IgG
Specificity
In 293HEK cells transfected with cds plasmid detects a band of 55kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein).
Purity/Purification
Epitope affinity purified
Form/Format
Polyclonal antibody supplied as a 100ul (2.5mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
2mg/ml (varies by lot)
Applicable Applications for anti-tdTomato antibody
Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry-Frozen (IHC-F)
Application Notes
WB: 1:500-1:2,000
IF: 1:50-1:500
IHC-F: 1:50-1:1,000
IF: 1:50-1:500
IHC-F: 1:50-1:1,000
Conjugation
DyLight633
Immunogen
Purified recombinant peptide produced in E Coli
Preparation and Storage
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Reactivity Chart
Reactivity Chart
Immunofluorescence (IF)
(Immunofluorescence in Drosophila larvae expressing tdTomato fusion protein in neurons usingAnti-tdTomato conjugated to DyLight633 at 1/500;)
Immunofluorescence (IF)
(Immunofluorescence in Drosophila larvae expressing tdTomato fusion protein in neurons usingAnti-tdTomato conjugated to DyLight633 at 1/500;)
Testing Data
(Anti-tdTomato Ab conjugated to DyLight 633 at 1/2,500 dilution using HEK293 transfected cell lysates at 50 ug per lane;)
Testing Data
(Anti-tdTomato Ab conjugated to DyLight 633 at 1/2,500 dilution using HEK293 transfected cell lysates at 50 ug per lane;)
Related Product Information for anti-tdTomato antibody
Goat polyclonal antibody to tdTomato (red fluorescent protein) conjugated to DyLight 633. tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation. It´s brightness and emission wavelength, makes it ideal for live animal research.
Product Categories/Family for anti-tdTomato antibody
Similar Products
Product Notes
The tdTomato (Catalog #AAA448225) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Anti-tdTomato, DyLight633 reacts with Transfected cells, tdTomato, mCherry, red fluorescent protein (DsRed) from so-called disc corals of Discosoma species. and may cross-react with other species as described in the data sheet. AAA Biotech's tdTomato can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry-Frozen (IHC-F). WB: 1:500-1:2,000 IF: 1:50-1:500 IHC-F: 1:50-1:1,000. Researchers should empirically determine the suitability of the tdTomato for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "tdTomato, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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