Rabbit respiratory syncytial virus (RSV) Fusion glycoprotein/RSV-F Monoclonal Antibody | anti-RSV-F antibody

Human respiratory syncytial virus (RSV) Fusion glycoprotein/RSV-F Neutralizing Antibody

Cat. Num. AAA8104701

• Reactivity: RSV
• Applications: ELISA
• Purity: Protein A
• Synonyms: respiratory syncytial virus (RSV) Fusion glycoprotein/RSV-F; Monoclonal Antibody; Human respiratory syncytial virus (RSV) Fusion glycoprotein/RSV-F Neutralizing Antibody; Anti-F Antibody; Anti-HRSVgp08 Antibody; anti-RSV-F antibody

• Host: Rabbit
• Reactivity: RSV
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: R338
• Specificity: Human respiratory syncytial virus (RSV) Fusion glycoprotein/RSV-F.; Has cross-reactivity in ELISA with recomibnantF protein of A type of long strain (40039-V08B), A2 strain (11049-V08B), RSS2 strain (40037-V08B), B type of B1 strain; Has cross-reactivity in ELISA with recomibnantF protein of A type of long strain (40039-V08B), A2 strain (11049-V08B), RSS2 strain (40037-V08B), B type of B1 strain
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-RSV-F antibody: ELISA (EIA) (Cap), Microneutralizaiton (MN)
• Application Notes: Antibody's applications have not been validated with corresponding viruses.; ELISA (Cap): This antibody will detect Human respiratory syncytial virus (RSV) (A2) Fusion glycoprotein/RSV-F in ELISA pair set.; In a sandwich ELISA, it can be used as capture antibody when paired with.
• Immunogen: Recombinant Human respiratory syncytial virus (RSV) Fusion glycoprotein/RSV-F protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human respiratory syncytial virus (RSV) Fusion glycoprotein/RSV-F and was produced using recombinant antibody technology.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Testing Data

(The neutralization activity of RSV F protein antibody is Measured by Microneutralization test in vitro. The cytopathic effect of VERO cells induced by 100 pfu of RSV A-A2 strain is neutralized by increasing concentrations of anti RSV Monoclonal Antibody. The neutralizing titer (IC50) of antibody is 0.6-2.3 ug/mL. Data is provided by the customer. We has not validated this application.)

Related Product Information for anti-RSV-F antibody

Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. It is classified within the genus pneumovirus of the family paramyxoviridae. Like other members of the family, HRSV has two major surface glycoproteins (G and F) that play important roles in the initial stages of the infectious cycle. The G protein mediates attachment of the virus to cell surface receptors, while the F protein promotes fusion of the viral and cellular membranes, allowing entry of the virus ribonucleoprotein into the cell cytoplasm. The fusion (F) protein of RSV is synthesized as a nonfusogenic precursor protein (F), which during its migration to the cell surface is activated by cleavage into the disulfide-linked F1 and F2 subunits. This fusion is pH independent and occurs directly at the outer cell membrane, and the F2 subunit was identifed as the major determinant of RSV host cell specificity. The trimer of F1-F2 interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and induces the fusion between host cell and virion membranes. Notably, RSV fusion protein is unique in that it is able to interact directly with heparan sulfate and therefore is sufficient for virus infection. Furthermore, the fusion protein is also able to trigger p53-dependent apoptosis.

References

Martin-Gallardo A. et al., 1993, J Gen Virol. 74 (3): 453-8. Jose A M. et al., 1997, J Gen Virol. 78: 2411-8. Feldman SA. et al., 1999, J Virol. 73 (8): 6610-7. Zlateva K.T. et al., 2004, J Virol. 78 (9): 4675-83. Trento A. et al., 2006, J Virol. 80 (2): 975-84. Branigan P J. et al., 2006, J Gen Virol. 87 (2): 395-8. Eckardt-Michel J. et al., 2008, J. Virol. 82: 3236-49.

NCBI and Uniprot Product Information

• NCBI GI #: 138251
• UniProt Accession #: P03420; P88811
• Molecular Weight: 63,453 Da
• NCBI Official Full Name: Fusion glycoprotein F0
• UniProt Protein Name: Fusion glycoprotein F0
• UniProt Gene Name: F
• UniProt Synonym Gene Names: Protein F
• UniProt Entry Name: FUS_HRSVA

Uniprot Description

During virus entry, induces fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm. The fusogenic activity is inactive untill entry into host cell endosome, where a furin-like protease cleaves off a small peptide between F1 and F2 (PubMed:18216092). Interacts directly with heparan sulfate and may participates in virus attachment (PubMed:10864656). Furthermore, the F2 subunit was identifed as the major determinant of RSV host cell specificity (PubMed:11493675). Later in infection, proteins F expressed at the plasma membrane of infected cells can mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis. The fusion protein is also able to trigger p53-dependent apoptosis (PubMed:12663767).