RSV Fusion protein/RSV-F (Strain RSS-2) Protein Recombinant Protein | RSV-F recombinant protein

Recombinant RSV Fusion protein/RSV-F (Strain RSS-2) Protein (His Tag)

Cat. Num. AAA2569563

• Purity: >95% as determined by SDS-PAGE
• Synonyms: RSV Fusion protein/RSV-F (Strain RSS-2) Protein; Recombinant RSV Fusion protein/RSV-F (Strain RSS-2) Protein (His Tag); F Protein; RSV; HRSVgp08 Protein; Human respiratory syncytial virus; RSV-F recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4, 10% gly. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
• Sequence: Met 1-Thr 529

• Species: RSV
• Tag: C-His
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Protein Construction: A DNA sequence encoding the extracellular domain of human RSV (strain RSS-2) fusion protein (P11209)(Met 1-Thr 529) was expressed with a polyhistidine tag at the C-terminus.
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; This product is provided as lyophilized powder which is shipped with ice packs.

SDS-Page

Related Product Information for RSV-F recombinant protein

Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. It is classified within the genus pneumovirus of the family paramyxoviridae. Like other members of the family, HRSV has two major surface glycoproteins (G and F) that play important roles in the initial stages of the infectious cycle. The G protein mediates attachment of the virus to cell surface receptors, while the F protein promotes fusion of the viral and cellular membranes, allowing entry of the virus ribonucleoprotein into the cell cytoplasm. The fusion (F) protein of RSV is synthesized as a nonfusogenic precursor protein (F), which during its migration to the cell surface is activated by cleavage into the disulfide-linked F1 and F2 subunits. This fusion is pH independent and occurs directly at the outer cell membrane, and the F2 subunit was identifed as the major determinant of RSV host cell specificity. The trimer of F1-F2 interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and induces the fusion between host cell and virion membranes. Notably, RSV fusion protein is unique in that it is able to interact directly with heparan sulfate and therefore is sufficient for virus infection. Furthermore, the fusion protein is also able to trigger p53-dependent apoptosis.

Product Categories/Family for RSV-F recombinant protein

Virus related Protein

NCBI and Uniprot Product Information

• NCBI GI #: 1353201
• UniProt Accession #: P11209
• Molecular Weight: 63,334 Da
• NCBI Official Full Name: Fusion glycoprotein F0
• UniProt Protein Name: Fusion glycoprotein F0
• UniProt Gene Name: F

Uniprot Description

During virus entry, induces fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm. The fusogenic activity is inactive untill entry into host cell endosome, where a furin-like protease cleaves off a small peptide between F1 and F2. Interacts directly with heparan sulfate and may participate in virus attachment. Furthermore, the F2 subunit was identifed as the major determinant of RSV host cell specificity. Later in infection, proteins F expressed at the plasma membrane of infected cells can mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis. The fusion protein is also able to trigger p53-dependent apoptosis.