Rabbit anti-Cynomolgus RBP4 Polyclonal Antibody | anti-RBP4 antibody

Anti-RBP4 Antibody, Rabbit Polyclonal

Cat. Num. AAA8113260

• Reactivity: Cynomolgus
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: RBP4; Polyclonal Antibody; Anti-RBP4 Antibody; Rabbit Polyclonal; RBP4 Antibody; Rabbit PAb; Antigen Affinity Purified; retinol binding protein 4; plasma; anti-RBP4 antibody

• Host: Rabbit
• Reactivity: Cynomolgus
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Cynomolgus RBP4
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-RBP4 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IHC-P: 1:1000-1:4000; IP: 0.5-2uL/mg of lysate
• Immunogen: Recombinant Cynomolgus RBP4 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Cynomolgus RBP4. RBP4 specific IgG was purified by Cynomolgus RBP4 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of cynomolgus RBP4 in cynomolgus liver with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

Immunoprecipitation (IP)

(cyno RBP4 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-cyno RBP4 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-cyno RBP4 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size: 23 kDa)

Western Blot (WB)

(Anti-RBP4 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:23 kDaObserved band size:23 kDa)

Related Product Information for anti-RBP4 antibody

Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A), and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily, RBP4 containing a beta-barrel structure with a well-defined cavity is secreted from the liver, and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes, and its deficiency affects night vision largely. Recently, RBP4 as an adipokine, is found to be expressed in adipose tissue and correlated with obesity, insulin resistance (IR) and type 2 diabetes (T2DM).

References

Yang Q, et al. (2005) Serum retinol binding protein 4 contributes to insulin resistance in obesity and type 2 diabetes. Nature. 436(7049): 356-62.Choi SH, et al. (2008) High plasma retinol binding protein-4 and low plasma adiponectin concentrations are associated with severity of glucose intolerance in women with previous gestational diabetes mellitus. J Clin Endocrinol Metab. 93(8): 3142-8.Tepper BJ, et al. (2010) Serum retinol-binding protein 4 (RBP4) and retinol in a cohort of borderline obese women with and without gestational diabetes. Clin Biochem. 43(3): 320-3.